Porous-layer Open Tubular
Porous-layer Open Tubular - Related Videos
01:45
Vesicular Tubular Clusters
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2023
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi. With the help of motor proteins such...
01:28
Tubular Reabsorption and Secretion
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2025
Tubular secretion and reabsorption are two critical processes in the nephron tubule of the kidneys. When the fluid filtered from the glomerulus enters the proximal convoluted tubule, it is referred to as filtrate, and its composition changes due to tubular reabsorption and secretion. Tubular reabsorption is a selective process that starts when the filtrate enters the proximal tubules. It involves substances traveling through the transcellular route (through the tubule cell and peritubular...
03:21
Murine Renal Cell Isolation: A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells
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2023
In this video, we demonstrate the steps to isolate and purify renal tubular epithelial cells for a wide range of molecular biology and cell culture studies.
09:05
Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
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Cited by 9 •
2015
Nitrogen is an effective supercritical fluid for extraction or drying processes due to its small molecular size, high density in the near-liquid supercritical regime, and chemical inertness. We present a supercritical nitrogen drying protocol for the purification treatment of reactive, porous materials.
06:32
Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions
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2025
Here, we describe the protocols for obtaining primary cultures of human renal tubular epithelial cells (HRTEC) from the kidney cortex to develop monolayers and three-dimensional cultures on an extracellular matrix (3D-HRTEC).
FAQs
Retention differences arise from how strongly and in what manner each compound interacts with the porous stationary layer. Adsorption, molecular size, polarity, and volatility can each influence the time an analyte remains associated with the layer rather than moving with the carrier gas. Because compounds respond differently to these factors, retention times can distinguish constituents within a mixture.
The porous stationary layer provides the surface with which analytes interact during their passage through the column. Its interactions can reflect adsorption, molecular size, polarity, or volatility, causing compounds to spend different amounts of time in the stationary region. This unequal retention creates separation, allowing chemically different constituents to produce distinct retention times.
The unobstructed central channel lets the carrier gas move through the column with relatively low resistance. Analytes therefore encounter a direct gas-flow pathway while still contacting the porous layer on the inner wall. This arrangement supports efficient mass transfer, helping compounds move and equilibrate effectively enough to produce sensitive separations of volatile and semi-volatile substances.
The separation is represented by the retention time associated with each compound. As the carrier gas transports analytes through the open channel, different interactions with the porous layer delay compounds by different amounts. Comparing these distinct times helps reveal separate constituents in a chemical mixture, even when the original sample contains many volatile or semi-volatile substances.
These columns are suited to volatile and semi-volatile substances that can be transported by a carrier gas and interact with the porous layer. They are particularly useful when a sample contains multiple constituents requiring separation before characterization. Relevant sample contexts include environmental, petrochemical, food, and biological research, where complex chemical mixtures are commonly examined.
They provide separation patterns based on the retention times of individual constituents. Those times reflect differing interactions with the porous layer and can therefore help characterize the chemical composition of a mixture. In chemistry research, this information supports analysis of complex samples from environmental, petrochemical, food, and biological settings, especially when volatile or semi-volatile compounds are present.