Indentation Mode
Indentation Mode - Related Videos
08:21
Quantitative Hardness Measurement by Instrumented AFM-indentation
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Cited by 6 •
2016
This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.
07:07
Biomechanical Characterization of Human Soft Tissues Using Indentation and Tensile Testing
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Cited by 111 •
2016
Tissue biomechanics is important for maintaining cell shape and function and for determining phenotype. This report demonstrates non-destructive mechanical protocols for characterizing elastic and viscoelastic properties of human soft tissues, which can be directly applied to tissue-engineered substrates to allow a close matching of engineered materials to native tissue.
11:19
Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry
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Cited by 36 •
2016
We present a set of techniques to characterize the viscoelastic mechanical properties of brain at the micro-, meso-, and macro-scales.
01:07
What is a Mode?
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2023
The mode is one of the commonly used measures of a central tendency. It is defined as the most frequent value in a data set. There can be more than one mode in a data set if multiple values have the same highest frequency. For instance, suppose that the Statistics exam scores of 20 students are: 50; 53; 59; 59; 63; 63; 72; 72; 72; 72; 72; 76; 78; 81; 83; 84; 84; 84; 90; 93. Here, the mode is 72, as it occurs most frequently, five times. A data set with two modes is called bimodal. For example,...
02:44
Impact Indentation for Assessing the Mechanical Properties of a Mouse Brain Tissue
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2025
Source: Canovic, E. P., et. al., Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry. J. Vis. Exp. (2016)The video demonstrates using impact indentation to measure the mechanical properties of a hydrated mouse brain tissue, including stiffness, energy dissipation, and damping, through probe displacement and velocity analysis.
FAQs
It records how a sample deforms under a controlled load, producing a force-displacement response that can be analyzed for stiffness, elasticity, and viscoelastic behavior. Differences in these responses provide quantitative evidence that the mechanical state varies among cells, tissues, embryos, or developmental stages. The measurements therefore connect probe-surface interactions with mechanical changes during development over time.
A controlled load provides the input, while the resulting displacement captures how far the sample deforms. The recorded force-displacement relationship can then be examined for differences in stiffness, elasticity, or viscoelastic behavior. Because the load is controlled, measurements can support quantitative comparisons among biological samples examined during development.
Mechanical measurements are useful in morphogenesis because they connect measurable material behavior with developmental events. Tracking stiffness, elasticity, or viscoelastic behavior in cells, tissues, and embryos can help researchers relate changing mechanics to shape formation, differentiation, and coordinated development. The approach therefore adds a quantitative mechanical perspective to processes otherwise described by form or developmental state.
In an atomic force microscope, the probe is brought into controlled contact with the sample, a load is applied, and the resulting force-displacement response is recorded. Researchers then analyze that response to estimate mechanical properties. Applying the same measurement logic across cells, tissues, or embryos supports comparisons of mechanical state during growth and morphogenesis.
Indentation mode can be used to study cells, tissues, and embryos as they grow and undergo morphogenesis. Measurements can reveal whether their mechanical properties change across these developmental contexts, while the same approach also supports examination of mechanics associated with differentiation and coordinated development. This makes it useful when researchers need quantitative rather than purely descriptive mechanical information.
Results are most informative when interpreted as mechanical measurements tied to a sample and developmental context. A force-displacement response may be analyzed to estimate stiffness, elasticity, or viscoelastic behavior, then related to growth, morphogenesis, differentiation, or coordinated development. These comparisons provide a quantitative way to examine how mechanical properties change as development proceeds.