Streptavidin Reporter Dye
Streptavidin Reporter Dye - Related Videos
03:41
Analyzing Influenza Virus Internalization Using a Streptavidin Blocking Assay
0 Views •
2026
Source: Pohl, M. O. & Stertz, S. Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry. J. Vis. Exp. (2015)This video demonstrates the use of a streptavidin blocking assay to distinguish surface-bound from internalized influenza virus in human lung epithelial cells. Comparing fluorescence signals shows that a higher intensity after incubation indicates successful viral internalization.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
03:54
Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres
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2025
Source:Rhea Kang1, Gabby Mora1, Sherry Dunbar11Luminex, A DiaSorin Company, Austin.This video demonstrates multiplexed quantitative bacterial detection employing carboxylated magnetic polystyrene microspheres dyed into distinct sets. The procedure encompasses the formation of bead-bacteria complexes, magnetic separation, and flow-based analysis, enabling precise and simultaneous quantification of multiple bacteria in the same reaction well.
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
09:46
Synthesis of Plant Phenol-derived Polymeric Dyes for Direct or Mordant-based Hair Dyeing
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Cited by 12 •
2016
Here, we present a protocol to use pre-synthesized polymeric products derived from fungal laccase-catalyzed polymerization of plant phenols, either with or without mordant agents (e.g., FeSO4), to induce detergent-resistant keratin hair dyeing within 2.5 hours.
FAQs
The tetrameric protein presents multiple high-affinity binding sites for biotin, so one labeled streptavidin reagent can associate with several biotin-dependent assay components. This multivalent arrangement supports signal amplification compared with a single labeling event. In practice, it helps make low-abundance immune markers, microbial antigens, or pathogen-associated nucleic acids more readily detectable.
A biotin-streptavidin bridge separates target recognition from signal generation. Researchers can use a biotinylated antibody, nucleic acid probe, or other targeting component, then add the dye-conjugated reagent as a detection step. This modular design provides flexible probe selection and avoids requiring the primary targeting molecule itself to carry the fluorescent or color-producing label.
The attached reporter determines the type of detectable output. Fluorescent labels support visualization by microscopy or measurement in flow cytometry, whereas color-based labels support signal assessment in immunoassay formats. Selecting the reporter according to the assay platform allows the same biotin-dependent binding principle to serve different detection strategies in immunology and infection research.
High-affinity recognition provides a selective connection between the biotinylated assay component and the labeled streptavidin reagent. Because the reporter is recruited through this defined molecular interaction, the resulting signal remains linked to the antibody, probe, or other component chosen for target recognition. This supports sensitive detection while preserving flexible assay design.
A typical workflow places a biotinylated antibody, nucleic acid probe, or related assay component at the target of interest, followed by addition of the dye-conjugated streptavidin reagent. Binding connects the target-recognition component to the detectable reporter. The resulting fluorescent or color-based signal can then be examined using the instrument or imaging format selected for the assay.
The system is useful when experiments must identify immune cells, microbial antigens, or pathogen-associated nucleic acids through biotinylated recognition components. Its format supports indirect immunofluorescence, flow cytometry, microscopy, and immunoassays. Researchers can therefore adapt the same binding strategy to cellular analysis, microscopic localization, or broader detection of infection-related molecular targets.