Ex Vivo Preservation
Ex Vivo Preservation - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
09:01
Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Pancreas Allografts before Transplantation
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Cited by 3 •
2022
Normothermic ex vivo machine perfusion (NEVP) has scarcely been explored for the preservation of pancreas allografts. We present an innovative preservation technique for pancreas allografts before transplantation.
08:15
Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
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2025
Donor heart preservation is a major determinant of success in heart transplantation. Technological advances have led to optimizing the conditions of preservation, along with a more accurate assessment of the donor heart before transplantation. We herein describe two innovative technologies currently applied for cardiac preservation: uniform cooling preservation and normothermic ex-situ heart perfusion.
08:07
Regulating Temperature in the Lab: Preserving Samples Using Cold
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2023
Preservation of laboratory samples, specimens, and reagents using extreme cold is routinely performed in biomedical research labs. This video will discuss some of the methods for keeping laboratory samples cold and will explain the correct cooling method to use for each experimental requirement. For example, cooling agents, such as ice and dry ice, are typically used when keeping samples cold during experiments. This video discusses the physical properties of the most commonly used cooling...
01:28
Pasteurization and Food Preservation
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2026
Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
FAQs
Temperature control reduces the rate of metabolic deterioration while biological material remains outside the body. Maintaining an appropriate temperature can therefore help limit cellular injury and retain structure and function for a longer period. In medicine, this expands the time available for transport, evaluation, or preparation before transplantation, tissue banking, or laboratory study.
Oxygen availability and nutrient supply are controlled together because preserved cells and tissues must retain conditions that support viability while metabolic deterioration is slowed. An imbalance can compromise the ability to maintain cellular function even when other conditions are suitable. Coordinating these variables is therefore important when preserving organs, tissues, or cells for later medical use or investigation.
Fluid balance helps maintain the physical and functional condition of cells, tissues, organs, and specimens during storage outside the body. It is one of the controlled conditions used alongside temperature, oxygen availability, nutrient supply, and sterility. Maintaining this balance supports efforts to limit cellular injury and preserve usable structure and function for subsequent evaluation or application.
A preservation workflow controls temperature, oxygen availability, nutrient supply, sterility, and fluid balance. These variables are adjusted to slow metabolic deterioration, limit cellular injury, and retain biological function. The relevant combination depends on whether the material is a cell, tissue, organ, or specimen, with the goal of extending the period available for transport, evaluation, or study.
Ex Vivo Preservation is especially useful when biological material must remain viable long enough for transplantation, tissue banking, cell-based therapy, surgical planning, or biomedical research. By extending the time available for transport and evaluation, it can support more deliberate handling of valuable material. The approach may also improve graft quality and reduce waste in transplantation-related workflows.
Preserving human tissues and other biological specimens outside the body enables their structure and function to be retained for controlled laboratory investigation or preparation before clinical use. In biomedical research, this supports more reliable study of human tissues under controlled conditions. For surgical planning, preserved material can provide time for evaluation before an intervention, while cell-based therapies benefit from maintained cellular viability.