Fat Pad Aspiration
Fat Pad Aspiration - Related Videos
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
06:41
Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads
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2023
This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
09:41
Performing and Processing FNA of Anterior Fat Pad for Amyloid
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Cited by 28 •
2010
Fat pad aspiration is a preferred, minimally invasive, and low cost approach as compared to other methods to detect amyloid for diagnosis of systemic amyloidosis. This video article demonstrates a procedural outline for performing fat pad aspiration with appropriate processing of the specimen for the optimal diagnostic outcome.
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
FAQs
During sampling, suction removes adipose cells together with surrounding tissue, preserving material that can be examined in the laboratory. Prepared specimens may undergo staining or other analyses designed to reveal abnormal deposits such as amyloid. This approach links a readily collected subcutaneous sample to evidence of disease processes that may affect tissues or organs elsewhere in the body.
Abdominal subcutaneous fat can provide tissue for laboratory examination without requiring direct sampling of an organ suspected of involvement. This makes the procedure an alternative to an affected-organ biopsy when clinicians are evaluating systemic disease, including systemic amyloidosis. The result can therefore support diagnosis while reducing the need to obtain tissue from a potentially involved organ.
Laboratory preparation determines how the collected tissue is examined for abnormal material. Stains can make deposits such as amyloid identifiable, while other analyses may provide additional characterization of the specimen. These findings do not stand alone; clinicians interpret them together with the patient’s clinical findings and results from other laboratory or imaging tests.
The procedure begins with local anesthesia at the sampling site. A needle attached to a syringe is then used to apply suction and remove a small amount of subcutaneous adipose tissue with surrounding material. After collection, the specimen is prepared for stains or other laboratory analyses, allowing the tissue to be assessed for abnormal deposits.
Clinicians may consider abdominal sampling when evaluating suspected systemic amyloidosis or another systemic disease in which abnormal deposits might be present in subcutaneous fat. Its value comes from providing tissue for examination through an alternative to sampling an affected organ. The findings are incorporated into a broader diagnostic assessment rather than used in isolation.
The laboratory observation is only one part of the diagnostic process. Evidence of an abnormal deposit in the prepared fat specimen can support the clinical evaluation, but interpretation must be combined with symptoms or other clinical findings, laboratory results, and imaging information. This integrated approach helps place the tissue finding in the context of suspected systemic disease.