Mouse Cardiac Models
Mouse Cardiac Models - Related Videos
10:25
Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury
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Cited by 20 •
2011
A powerful model for perioperative and critical care related acute kidney injury is presented. Using whole body hypoperfusion induced by cardiac arrest it is possible to nearly replicate the histologic and functional changes of clinical AKI.
09:05
Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging
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Cited by 25 •
2016
The goal of this protocol is to noninvasively assess cardiac structural and functional changes in a mouse model of heart disease created by transverse aortic constriction, using B- and M-mode echocardiography and color/pulse wave Doppler imaging.
10:22
Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse
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Cited by 29 •
2017
Echocardiographic examination is frequently used in mice. Expensive high-resolution ultrasound devices have been developed for this purpose. This protocol describes an affordable echocardiographic procedure combined with histological morphometric analyses to determine cardiac morphology.
03:02
Immunostaining of Bacteria-Filled Microlesions in a Mouse Cardiac Tissue Section for Imaging
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2026
Source: Brown, A. O., and Orihuela, C. J. Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling. J. Vis. Exp. (2015)The video demonstrates the immunostaining of chemically fixed mouse cardiac tissue sections to visualize pathogenic bacteria localized within infection-induced microlesions. The process includes tissue washing, membrane permeabilization, blocking of nonspecific sites, antibody labeling, nuclear counterstaining, and confocal...
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
FAQs
Genetic modification can alter cardiac biology in a defined way, while interventions such as myocardial injury or drug exposure create a controlled experimental challenge. Researchers can then compare resulting changes in contractility, electrical activity, remodeling, or tissue pathology. This combination helps separate disease-related mechanisms from responses caused by the intervention itself and supports more focused evaluation of potential therapeutic targets.
Contractility indicates how effectively the heart performs its mechanical function, whereas electrical activity provides information about cardiac signaling and rhythm-related changes. Remodeling captures alterations that develop in cardiac structure or organization, and tissue pathology reveals changes at the tissue level. Considering these outcomes together gives a broader assessment than relying on a single measure of cardiac dysfunction.
Standardized genetics reduce biological variation between experimental groups, making observed differences easier to associate with a genetic modification, intervention, or treatment. Manageable experimental conditions further support controlled comparisons across studies. This consistency is important when researchers examine disease mechanisms, assess treatment efficacy, or interpret potential toxicity, because differences in experimental background can otherwise complicate conclusions.
A typical workflow combines a defined genetic background or modification with a controlled intervention, followed by measurement of relevant cardiac outcomes. Depending on the research question, investigators may examine contractility, electrical activity, remodeling, and tissue pathology. Comparing these findings with appropriate experimental groups helps reveal how the disease process develops and how the heart responds to treatment or injury.
These models are useful when researchers need to connect a cardiac condition with measurable changes in heart function, electrical behavior, structural remodeling, or tissue pathology. Heart failure, ischemia, and arrhythmia can therefore be examined through different outcome profiles rather than a single endpoint. The resulting comparisons help clarify disease mechanisms and identify features that may respond to intervention.
Researchers can use mouse cardiac models to evaluate therapeutic targets, treatment efficacy, and potential toxicity within controlled experimental conditions. Results may show whether an intervention changes relevant functional, electrical, structural, or tissue outcomes. Because the models support standardized comparisons, their findings can help guide subsequent translational research, while also indicating which treatment effects or risks require further investigation.