Young Old Mouse
Young Old Mouse - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
08:24
Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach
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2016
This protocol describes a method to quantify mouse sociability. Mice are videotaped as they move and interact in a special cage. Movie processing allows for the automated quantification of sociability with excellent accuracy and reliability.
06:42
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
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Cited by 29 •
2011
In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
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Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
FAQs
Age-defined comparisons are valuable because they hold a major experimental variable, age, relatively constant while researchers examine biological differences. This design allows cellular function, tissue regeneration, immune activity, and inflammation to be assessed across lifespan stages. The resulting contrasts can reveal processes associated with functional decline rather than merely documenting that older animals perform differently.
Differences may reflect altered cellular function, reduced tissue regeneration, changes in immune activity, or increased inflammation. Examining these processes together helps connect age-associated changes across biological systems instead of treating decline as an isolated outcome. Such analyses can also identify features linked with resilience, meaning preserved function, or with rejuvenation after an intervention.
Researchers compare functional responses, not only baseline differences, between age groups. Responses to injury or treatment can show whether older animals retain particular capacities, display impaired recovery, or regain age-associated functions after intervention. This approach links observed outcomes to potential mechanisms of resilience or rejuvenation and helps assess whether a candidate treatment changes the aging-related phenotype.
A typical comparison begins with age-defined young and old mouse groups, followed by assessment of a selected biological system or response. Researchers may measure cellular function, regeneration, immune activity, inflammation, or recovery after injury. They then compare the groups and, when testing an intervention, examine whether treatment-associated changes differ by age and restore impaired functions.
The studies can reveal how aging relates to functional decline in cells, tissues, immune responses, or recovery processes. They may also identify biological features associated with preserved performance in older animals. When an intervention is included, comparisons indicate whether a candidate treatment influences age-associated dysfunction and whether its effects are consistent with restoration of a measured function.
These models are useful when researchers need to examine age-related disease or treatment responses under controlled conditions. Applications described for this approach include neurodegeneration, impaired healing, and metabolic disorders. The same comparisons support evaluation of therapeutic interventions by showing whether an experimental treatment improves functions that differ between young and old animals.