750 Nanometer Laser
750 Nanometer Laser - Related Videos
11:56
Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Cited by 11 •
2014
Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.
10:25
Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
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Cited by 39 •
2016
We present a method for achieving sub-nanometer resolution images with amplitude-modulation (tapping mode) atomic force microscopy in liquid. The method is demonstrated on commercial atomic force microscopes. We explain the rationale behind our choices of parameters and suggest strategies for resolution optimization.
14:18
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
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Cited by 2 •
2016
A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.
09:59
An “All-laser” Endothelial Transplant
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Cited by 5 •
2015
An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.
12:54
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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Cited by 13 •
2016
Coherent anti-Stokes Raman scattering (CARS) microscopy based on inherent vibration of molecule bonds permits label-free chemically selective live cell imaging. This work presents the implementation of a complementary microscopy technique on a standard multiphoton laser scanning microscope based on a femtosecond Ti:sapphire laser and an OPO laser.
FAQs
Stimulated emission produces photons with matching wavelength and organized phase, giving the beam coherence and narrow spectral bandwidth. These properties help researchers deliver controlled illumination to optical systems rather than broad, mixed-wavelength light. The resulting spectral precision can support experiments in which fluorescent probes or light-sensitive molecules respond selectively to the applied radiation.
Wavelength determines photon energy and influences how light travels through tissue and interacts with fluorescent or light-sensitive molecules. Consequently, choosing 750 nanometer illumination affects whether an optical setup is suitable for fluorescence excitation, neural recording, or controlled photostimulation. The wavelength therefore links laser selection to both molecular response and the depth or distribution of illumination.
Power, pulse duration, and beam delivery determine how much illumination reaches the biological target, for how long, and across what spatial pattern. Adjusting these variables allows researchers to balance spatial resolution with biological exposure. The same wavelength can therefore support different experimental conditions, from localized stimulation to optical measurements of activity, depending on the chosen settings.
The probe or light-sensitive molecule determines how incoming 750 nanometer light is converted into an observable or biological response. If the relevant molecule supports fluorescence, the illumination can contribute to optical recording; if it responds functionally to light, the same source may support photostimulation. Instrumentation and probe properties must therefore be considered together rather than selecting wavelength alone.
A basic workflow begins by matching the wavelength to the fluorescent or light-sensitive probe and the intended measurement or stimulation. Researchers then configure laser power and pulse duration, establish beam delivery through the optical system, and adjust the illumination pattern for the desired spatial resolution. These choices help manage biological exposure while targeting neural circuits, cells, or activity measurements.
In neuroscience, 750 nanometer illumination can contribute to studies of neural circuits, cellular activity, and brain function. Depending on the probes and instrumentation, researchers may use it for fluorescence excitation, neural recording, or controlled photostimulation. The resulting optical measurements or responses provide experimental information about neural activity and circuit behavior under defined illumination conditions.