Micro-pump Injection

Micro-pump injection is a controlled drug-delivery method that uses a small mechanical pump to administer precise volumes of fluid over a defined period. In neuroscience, the pump connects to a catheter or cannula, often positioned stereotaxically in a specific brain region or near the spinal cord, while programmed flow rates regulate infusion and limit dosing variability. This approach supports localized delivery of pharmacological agents, neurotransmitter modulators, or other experimental solutions with less repeated handling than manual injections. By improving temporal and spatial control, micro-pump injection helps researchers investigate neural circuits, evaluate drug effects, and model neurological conditions.

Micro-pump Injection - Related Videos

Research

JoVE Journal - Neuroscience

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

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Cited by 3 •

2015

Here, we craft a glass pipette with dual functions: inhibition of deep brain structures by microinjections of drugs and real-time monitoring of their effects through simultaneous electrophysiological recordings.

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

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Cited by 7 •

2017

Here, we present a protocol to describe the use of neurotrophin 4 (NTF4) systemically and directly to remodel rat aging laryngeal muscles.

Continuous Drug Infusion System in Mouse Model: A Surgical Procedure to Implant Micro-osmotic Pump Infusion System in the Mouse Brain for Continuous Drug Delivery

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2025

In this video, we demonstrate the assembly of a micro-osmotic pump brain infusion system followed by its surgical implantation in the mouse skull. This system enables continuous delivery of potential drugs and facilitates the study of their effects on the brain and brain tissue.

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

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Cited by 12 •

2013

Micro-particle image velocimetry (μPIV) is used to visualize paired images of micro particles seeded in blood flows which are cross-correlated to give an accurate velocity profile. Shear rate, maximum velocity, velocity profile shape, and flow rate, each of which has clinical applications, can be derived from these measurements.

Education

JoVE Core - Cell Biology

ATP Driven Pumps III: V-type Pumps

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2023

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...

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