来源: 凯斯图尔特, RVT, RLATG, CMAR;瓦莱丽. 施罗德, RVT, RLATG。圣母大学, #160;
已经证明, 即使是最小的老鼠和老鼠的处理对动物也有压力。处理笼子改变和其他无创程序导致心率、血压和其他生理参数, 如血清皮质酮水平的增加。波动可持续数小时。注射和采血所需的克制方法也会导致可能影响科学数据的生理变化。需要对老鼠和老鼠进行适当的训练, 以尽量减少对动物的影响。 1 小鼠和大鼠可以用约束装置或化学试剂手动约束。本手稿涵盖了手动方法和约束装置的使用。所有的限制方法包括把动物从笼子里抬起来的过程.
从笼子里取出老鼠或老鼠的常用方法包括用钳子将动物的尾巴抬起, 用管子或其他浓缩装置抓住尾巴的颈背或底部, 2 抓住周围的身体, 或舀入手掌的动物。 34
当用尾巴抬起一只老鼠或老鼠时, 必须把尾巴抓在靠近动物臀部的底部。如果动物从尾巴末端被举, 尾巴的皮肤能剥脱和被拉扯由身体重量的重音;脱的损伤会导致尾部被截肢。 3 将动物从一个笼子移到另一笼中, 或移动到工作表面, 应该迅速而稳定地进行。将动物悬挂在空气中的任何时间, 不仅会引起窘迫, 而且还会使动物扭动或挣扎, 可能造成对尾部的伤害。处理者不应该把他们的手放在动物下面, 因为它是由它的尾巴解除的–它是本能的动物试图抓住手为安全, 造成叮咬.
在许多鼠标生产设备中, 钳子被用来把动物从笼子里移走。这是为生物安全, 因为它被认为有较少的机会, 从动物到动物的交叉污染。在处理个别动物或动物群之间, 应将镊子的尖端储存在酒精中。有各种各样的钳子用来抓老鼠。最常用的包括长敷料钳与创伤提示-或与橡胶, 塑料, 或硅胶管放置在镊子的两端, 以提供一个垫或舌钳橡胶夹具。 4
钳可以在颈部的颈背或尾部的底部抓住鼠标。大多数小鼠很快就习惯了这种方法, 并没有挣扎。然而, 耐心和实践是必要的变得熟练捉住动物。初学者将需要训练如何紧紧抓住老鼠, 特别是在颈背, 因为这可能会干扰呼吸。在尾巴上使用镊子时, 必须小心把钳子放在尾巴底部附近, 以避免受伤.
对于焦虑或陈列影响的动物, 把管子放进笼子可能会降低搬运难度, 减少对日常畜牧业的飞行/惊吓反应。由孔材料制成的管子可以加成浓缩物, 作为动物避难或寻求安全避难所的场所。这样的管子可以是开放式的或一端封顶的。大多数老鼠或老鼠会心甘情愿地进入管子, 当他们的笼子打开, 或者他们可以很容易地引导到管。一旦动物在试管内, 开放的一端被覆盖, 运送动物到新的位置。当放置在一个新鲜的笼子, 管可以轻轻地向上倾斜, 以鼓励动物离开它。动物很容易适应这种笼子的改变方法, 并会立即进入管, 使这种方法作为其他动物从一个地方移动到另一个快速和容易。虽然管不必须存在于家庭笼中是有效的, 有一个管在主机箱增加了对一个熟悉的安全站点的识别, 以及提供笼丰富。 2
一些机构更喜欢将动物举起来进行例行处理, 特别是处理老鼠。动物必须从小就习惯于这种方法。2-4 周龄的小鼠倾向于直跳。把一只手放在老鼠身上, #39 的背部会使它跳入处理程序的手掌, 让它被抓住。在把老鼠放进另一个笼子之前, 可能需要另一只手来防止它的蠕动。成年大鼠被轻轻地抓住胸腔周围, 举起, 并迅速放置到另一个笼子, 或在表面上.
当用小鼠或老鼠来改变笼子时, 经常需要在保持巢完好无损的情况下去除它们。使用两只手舀巢和新生儿从笼底, 完整的巢被移动到一个新的笼子。然而, 为了避免掉落幼崽, 每只手的手指必须紧紧地放在一起。一旦移动到一个新的笼子, 很重要的是要验证幼崽都存在。在移动它们之前和之后对幼崽进行计数是可取的。可以用塑料勺代替手。如果使用此方法, 应将手放在勺顶部, 以防止新生儿摆动或跳出勺。这种转移幼崽的方法, 与幼崽的个体处理相比, 对幼崽和大坝都不那么痛苦.
技术规程的限制要求有信心和坚定, 但温柔, 触摸。试探性的方法可能导致处理程序叮咬。如果处理反冲作为动物的尖叫, 该动物很快学会如何避免克制。然而, 攻击性的处理可能导致啮齿动物的受伤或死亡。一个自信而温和的方法的平衡是啮齿动物处理的目标.
老鼠和小老鼠可以通过抓住颈部颈背的皮肤来抑制, 称为 scruffing。这一方法的预防措施包括既抓皮肤太紧或过于松散。如果皮肤抓得太紧, 气道就会变得狭窄, 这会导致死亡。如果抓得太松, 动物将能够转动它的头并且可能咬住处理程序.
被搅动或极度恐惧的动物更容易叮咬。最好在处理之前先让动物平静下来。有各种各样的平静的技术尝试了以不同的成功。最可靠的方法是使用某种化学抑制, 通常是吸入麻醉剂。异氟醚或七氟醚是短效吸入麻醉药, 只有处理需要将动物从笼中转移到诱导室。一旦动物被麻醉 , 它们就可以纵或被人为地限制在手术过程中 .
使老鼠镇定的方法是把它裹在厚实的毛巾上, 让它藏在毛巾下面。把它放在实验室的外套袖子, 使他们可以埋葬他们的头在肘部的褶皱也可以提供一种安全的安全感的动物。同样的结果也可以通过把动物放在一个黑暗的区域里几分钟来实现.
1. Scruffing
小鼠最常使用 Scruffing 技术进行限制, 但幼鼠也可以用这种方法加以抑制。成年大鼠更难以抑制这种技术, 因为更肌肉颈部, 减少了松散的皮肤数量, 并反感这种方法.
图 1: 鼠标的单手和 two-handed 约束方法
2. 手套
有各种手套可供处理者穿戴以防止老鼠叮咬。由于失去了灵巧, 它们通常不适合与老鼠一起工作。经常, 许多手套类型的缺点胜过好处.
3。身体约束
图 2: 霸王龙抓地力和前肢交错法抑制大鼠
4。约束设备
图 3: 布鲁姆式抑制设备
图 4: 平底啮齿动物抑制剂
图 5: 管状抑制设备
图 6: 灵活的抑制剂
5。特定技术过程的约束方法
啮齿类动物被广泛使用模型生物体来研究各种科学问题, 并且已经证明, 即使是最小的处理也会给这些生物带来压力.
处理简单任务 (如保持笼的变化) 可能会导致心率、血压和血清皮质酮水平的升高, 并且这些波动可以持续几个小时。常规注射和血退程序所需的克制方法也可能导致生理变化, 这可能会影响科学数据。因此, 需要对老鼠和老鼠进行适当的训练, 以尽量减少对动物的影响.
在本视频中, 我们将首先回顾处理啮齿类动物的基本技术, 然后我们和 #39; 我将讨论啮齿动物的限制程序, 包括使用限制装置的人工技术和方法.
让与 #39; s 首先讨论啮齿类动物的基本处理。移动成年老鼠或老鼠的最常用方法之一是通过尾巴将动物抬起。在做这件事的时候, 必须在臀部附近的底部抓住尾巴。如果动物是由尾巴的末端举起, 皮肤可以被拉扯由于身体重量并且可能导致损伤导致尾巴截肢。重要的是, 将动物从一个地方移到另一处应该迅速而稳定地进行。将动物悬浮在空气中任何长度的时间, 不仅会引起窘迫, 而且会使动物扭动或挣扎, 可能对尾巴造成伤害。此外, 处理程序不应该把手放在动物下面, 因为它可能会试图抓住它的安全, 造成叮咬.
另一种处理成年啮齿动物的方法是使用镊子。常用的镊子类型是 long-dressing 钳或舌钳, 用橡胶、塑料或硅胶导管放置在两端以提供缓冲。钳可以抓住鼠标在颈背或底部的尾巴。钳子的使用最大限度地减少了动物和动物之间的交叉污染, 而且比在每个动物笼间更换手套要快和便宜。初学者将需要训练如何紧紧抓住小鼠用镊子, 特别是在颈背, 因为它可能会干扰呼吸。使用尾钳时, 必须注意将钳放在尾部底部的附近, 以避免受伤。记住, 在处理不同的动物群之间, 把它们面朝下存放在70% 乙醇中.
此外, 你可以使用由无渗透性材料制成的管子来转移动物。这些管子可以被添加到笼子里, 作为动物藏身或寻求安全避难所的地方。大多数老鼠或老鼠会心甘情愿地进入管子, 当他们的笼子打开, 或者他们可以很容易地引导入管子。当动物在里面时, 用手掌和手指盖住开放的末端。其次, 要鼓励动物离开, 在新的笼子里轻轻地把管子向上倾斜。动物很容易适应这种笼子的改变方法, 使它成为一个快速和简单的过程, 把动物从一个地方移动到另一个.
一些机构更喜欢动物–尤其是老鼠–被它们的身体提起, 即使是在例行处理的时候。成年大鼠被轻轻地抓住胸腔周围, 举起, 并迅速放置到另一个笼子。动物从小就习惯于这种处理方法, 这一点很重要。当和小鼠一起工作时, 把一只手放在老鼠身上, #39 的背部会使它跳入处理器的手掌, 让它被抓住。可能需要另一只手来防止老鼠从抓取中蠕动.
当用小鼠或老鼠来改变笼子时, 经常需要在保持巢完好无损的情况下去除它们。用两只手舀巢和幼崽从笼子底部到一个新的笼子。把你的手指紧紧地放在一起, 以免掉落幼崽。一旦巢已经移动到一个新的笼子, 重要的是要验证所有的幼崽都存在。或者, 一个塑料勺可以用来在它们的巢中移动新生啮齿动物。这样做, 重要的是, 你把你的手在顶部的勺子, 以防止新生儿摆动或跳出。与幼崽的个体处理相比, 铲整窝对幼崽和大坝都不那么痛苦.
现在我们已经讨论了啮齿动物处理的基础知识, 让与 #39; 我们深入研究了啮齿动物抑制的详细程序。首先, 我们将概述如何手动限制这些动物。使用单手或 two-handed 技术可以抑制小鼠和小鼠。然而, 对于成年大鼠来说, 使用霸王龙抓地力或前肢十字交叉法的全身克制是首选的技术.
让与 #39; 首先是学习 two-handed 约束方法。首先, 把动物放在他们能抓的表面上, 如铁丝条盖。用你占优势的手, 抓住尾巴在底部, 轻轻地拖着鼠标向后。这使动物保持在表面上, 并允许其身体伸展。接下来, 用你的非食指和拇指轻轻地把老鼠钉在头骨的底部, 抓住颈部松弛的皮肤, 把动物抬起来。用你剩余的手指来稳定动物的背部和拇指的底部。你可以通过把你的无名指钉在你的手掌上来保护后躯.
对于 one-handed 约束方法, 再次把动物放在它能抓的表面上。握住你的非手的第三和第四手指之间的尾巴, 并应用温柔的向后牵引。用你的食指和同一只手的拇指, 把啮齿动物钉在肩上。轻轻滑动你的拇指和食指向前, 抓住松散的皮肤在颈部和升降机的动物。使用你的中指, 以稳定的动物沿背部通过固定背部皮肤对基地的拇指.
我们将讨论的第三种约束方法是对老鼠的 two-handed 约束方法–霸王龙抓地力。首先, 把老鼠放在坚实的表面上, 像垫子一样, 它能抓牢。一方面, 用一只手握住尾巴底座, 另一方面把你的另一只手放在老鼠和 #39 上; 肩膀与你的食指和中指在两边分开。这是为了限制侧向头部运动。一旦头部稳定, 将身体围在前肢后面, 用中指和无名指和拇指环绕。避免压缩胸部, 以免它危及动物和 #39 的呼吸。最后, 如果你打算管理一个口腔胃, 抓住尾巴的底座而不抱后腿, 稳定尾巴和后腿;或与后腿保持, 如果意图是注入动物.
最后, 要用前肢纵横交错的方法来抑制老鼠, 把动物放在它能抓的表面上。然后用你的优势的手, 抓住尾巴在基地和应用温柔向后牵引, 使动物和 #39; 拉长的身体。接下来, 用你的另一只手, 将动物直接握在肩膀后面, 手指放在一侧, 拇指放在胸部的另一侧, 然后把手向前滑动。这项运动将迫使动物和 #39 的前肢在其下巴下交叉, 形成一种物理屏障, 防止动物把头向下移动。注意避免按压胸腔, 这会导致呼吸困难.
现在, 我们已经审查了手动约束技术, 让 & #39; s 学习如何使用专用设备来限制啮齿目动物.
我们 #39 的第一个设备; 我要学习的是布鲁姆式的克制装置。它有一个插槽, 沿着整个设备的长度和塑料鼻甲, 这是插入到保持动物到位。先松开螺丝, 取出鼻甲。然后, 定向约束管, 使狭缝朝上。抓住动物的尾巴底部, 轻轻拉动物进入克制装置, 后躯第一。一旦动物在里面, 滑动塑料鼻甲回管, 以防止它打开。将鼻甲, 使动物和 #39 的鼻子在开口处居中。不要把它放得太紧, 动物不能呼吸.
限制装置的另一个例子是 flat-bottomed 啮齿动物抑制剂, 它是一个半圆筒, 允许从顶部和底部进入设备的开口。它也有槽, 其中一个刚性塑料门可以插入, 以保持动物到位。要使用这个抑制剂, 抓住啮齿动物的尾巴底部与你的优势手, 并把它放在一个光滑的表面。用你的另一只手, 保持 flat-bottomed 约束管, 使开放的结束是在动物和 #39; s 头在45和 #176; 角度到表面。把管子放在动物和 #39 的头上, 然后立即敲击臀部以防止动物的背。一旦它在设备中, 滑动塑料门进入适当的插槽, 以确保动物和 #39; s 的位置.
我们 #39 的第三种设备; 我要讨论的是管子的限制。这些都是有机玻璃气缸与一个封闭的一端包含空气流通的孔, 并在开放的一端狭缝。使用管约束, 抓住动物, 并把它放在光滑的表面。接下来, 将管的开口端放置在45和 #176 的动物和 #39 的头部; 角度。一旦啮齿类动物进入试管, 点击臀部防止它的备份。最后 , 通过填料一胶手套 , 咬合打开 , 这将抓住有机玻璃表面 , 防止动物退出管 .
最后, 我们将讨论如何使用灵活的位, 这是一次性灵活的塑料锥可在不同的大小。锥体的选择取决于动物大小。理想情况下, 它应该足够长, 以延长2-3 英寸以外的动物和 #39 的臀部。象早先方法, 安置动物在光滑的表面和地方克制锥体在45和 #176; 角度在动物和 #39; s 头。一旦动物进入锥体, 抓住开放末端并且封印它迫使动物到末端。通过将塑料折叠到尾部的一侧, 并将粘合剂夹在折叠的塑料上, 以避免夹紧尾部或皮肤, 从而确保开口的安全.
在讨论了处理的基本知识和不同的限制技术之后, 让和 #39…..。
这些基本处理程序背后的知识和基本原理是维持动物和 #39 健康的核心。从动物诞生之日起, 他们就被简单的日常行为所操控ivities 喜欢称量, 检查和保持笼的变化, 这是应该执行, 无论科学的目标.
限制程序对于标记目的也很有用。例如, 为了执行像耳朵冲孔这样的程序, 动物需要受到 scruffing 的限制。另一方面, 在大鼠中插入微芯片时, 应使用一种约束装置, 这样它们就可以被永久性地标记.
最后, 手动限制方法用于一般注射程序, 如皮下和腹腔管理。在静脉注射时, 对抑制装置的认识是很有用的, 这就要求在注射过程中尽量减少动物的运动.
您和 #39; 我刚刚看了朱庇特和 #39. 对啮齿动物基本操作和约束技术的介绍。这些程序需要一个自信和坚定, 但温柔的触摸。试探性的方法可能会导致处理程序的叮咬, 而攻击性的处理可能会对啮齿类动物造成伤害。因此, 很重要的一点是要彻底学习这些技巧, 并找到一个自信而又温柔的把握的平衡点。一如既往, 感谢收看!
对笼子的改变和技术程序进行例行处理是实验动物应激的原因。虽然这种压力不是对动物整体福祉的威胁, 但它可以引起生理参数的波动, 从而对研究数据产生不利影响。使用熟练的人员, 适当的技术和设备可以减轻一些压力.
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Rodents are widely used model organisms to investigate diverse scientific questions, and it has been demonstrated that even minimal handling is stressful for these animals.
Handling for simple tasks such as cage changing may cause an increase in heart rate, blood pressure, and serum corticosterone levels, and these fluctuations can continue for several hours. The methods of restraint required for common injection and blood withdrawal procedures can also cause physiological changes, which can potentially affect the scientific data. Therefore, training for proper handling of mice and rats is required to minimize these effects on animals.
In this video, we will first review the basic techniques for handling rodents, and then we’ll discuss the rodent restraint procedures including manual techniques and methods involving usage of restraining devices.
Let’s begin by discussing the basics of rodent handling. One of the most common methods for moving either an adult mouse or rat is by lifting the animal by its tail. While doing this, it is imperative that the tail be grasped at the base near the rump. If the animal is lifted by the end of its tail, the skin can be pulled off due to the body weight and may lead to an injury resulting in tail amputation. Importantly, moving an animal from one place to another should be done quickly and steadily. Suspending the animal in air for any length time will not only cause distress, but will also cause the animal to twist or struggle, possibly causing harm to the tail. Also, the handler should not place a hand under the animal, because it may try to grasp it for security, resulting in a bite.
Another method for handling adult rodents is to use forceps. Types of forceps commonly used are long-dressing forceps or tongue forceps with rubber, plastic, or silicone tubing placed over the ends to provide a cushion. The forceps can grasp the mouse either at the scruff of the neck or at the base of the tail. The use of forceps minimizes cross contamination from animal to animal, and it is quicker and less expensive than changing gloves between each animal cage. Beginners will need to be trained on how tightly to grasp the mice with forceps, especially at the scruff as it could interfere with breathing. When using the forceps on the tail, care must be taken to place the forceps near the base of the tail to avoid injury. Remember to store them facedown in 70% ethanol between handling of different groups of animals.
Also, one can use tubes made of a non-porous material to transfer animals. These tubes can be added to a cage as a place for the animal to shelter or seek safe refuge. Most mice or rats would willingly enter the tubes when their cage is opened, or they can be easily guided into the tube. When the animal is inside, cover the open ends with your palm and fingers. Next, to encourage the animal to leave, gently tip the tube upward in the new cage. Animals are easily conditioned to this method of cage changing, making this a quick and easy process for moving animals from one place to another.
Some institutions prefer that animals-especially rats-be lifted by their body, even while routine handling. Adult rats are gently grasped around the thorax, lifted, and quickly placed into another cage. It is important that animals are habituated to this method of handling from an early age. When working with young rats, placing a hand over the rat’s back will cause it to jump into the palm of the handler allowing it to be grasped. A second hand may be needed to prevent the rat from squirming from the grasp.
When changing cages with neonate mice or rats, it is often necessary to remove them while keeping the nest intact. Use two hands to scoop the nest and pups from the bottom of the cage into a new cage. Hold your fingers close together to avoid dropping the pups. Once the nest has been moved to a new cage, it is important to verify that all the pups are present. Alternatively, a plastic scoop may be used to move neonate rodents in their nests. While doing this, it is important that you place your hand over the top of the scoop to prevent neonates from wiggling or jumping out. Compared to the individual handling of the pups, scooping the entire nest is less distressing to both the pups and the dams.
Now that we have discussed the basics of rodent handling, let’s delve into detailed procedures for rodent restraint. First we will outline how to restrain these animals manually. Mice and young rats can be restrained using the one handed or the two-handed technique. However, for adult rats, full body restraint using the T-rex grip or the forelimb crisscross method, are the preferred techniques.
Let’s begin by learning the two-handed restraint method. First, place the animal on a surface they can grip, such as the wire bar lid. With your dominant hand, hold the tail at the base and gently tug the mouse backwards. This causes the animal to hold onto the surface and allow its body to be stretched. Next, gently pin the rodent with your non-dominant index finger and thumb positioned at the base of the skull, and grasp the loose skin on the neck to lift up the animal. Use your remaining fingers to stabilize the animal with its back against the base of your thumb. You can secure the hindquarters by pinning the tail with your ring finger against the palm of your hand.
For the one-handed restraint method, again place the animal on a surface that it can grip. Hold the tail between the third and fourth fingers of your non-dominant hand and apply gentle backward traction. With your index finger and thumb of the same hand, pin the rodent over the shoulders. Gently slide your thumb and index finger forward and grasp the loose skin at the neck and lift the animal. Use your middle finger to stabilize the animal along the back by pinning the dorsal skin against the base of the thumb.
The third restraint method we will discuss is the T-rex grip- a two-handed restraint method for rats. First, place the rat on a solid surface, like a mat, that it can grip. While holding the base of the tail with one hand, place your other hand over the rat’s shoulder with your index and middle finger split on either side. This is to restrict the side-to-side head movement. Once the head is stabilized, encircle the body behind the forelegs with the middle and ring fingers and the thumb. Avoid compressing the chest so that it does not compromise the animal’s breathing. Finally, stabilize the tail and hindquarters by grasping the base of the tail WITHOUT holding onto the hind feet, if you plan to administer an oral gavage; or WITH holding onto the hind feet, if the intention is to inject the animal.
Lastly, to restrain a rat using the forelimb crisscross method, place the animal on a surface it can grip. Then with your dominant hand, hold the tail at the base and apply gentle backward traction so that the animal’s body elongates. Next, with your other hand, grasp the animal directly behind the shoulders, with your fingers on one side and thumb on the other side of the chest, and slide the hand forward. This motion will force the animal’s forelegs to cross under its chin, creating a physical barrier that prevents the animal from moving its head down. Take care to avoid compressing the chest, which will lead to difficulty in breathing.
Now that we have reviewed the manual restraint techniques, let’s learn how to restrain rodents using specialized devices.
The first device that we’ll learn about is the Broome-style restraint device. It has a slot that runs along the full length of the device and a plastic nosepiece, which is inserted to hold the animal in place. Start by loosening the screw to remove the nosepiece. Then, orient the restraint tube such that the slit is facing up. Grasp the animal at the base of the tail and gently pull the animal into the restraint device, hindquarters first. Once the animal is inside, slide the plastic nosepiece back into the tube to prevent it from opening. Place the nosepiece such that the animal’s nose is centered in the opening. Do not position it so tightly that the animal cannot breathe.
Another example of a restraint device is the flat-bottomed rodent restrainer, which is a half cylinder with openings that allow access from the top and the bottom of the device. It also has slots in which a rigid plastic gate can be inserted to hold the animal in place. To use this restrainer, grasp the rodent by the base of the tail with your dominant hand and place it on a smooth surface. Using your other hand, hold the flat-bottomed restraint tube so that the open end is at the animal’s head at a 45° angle to the surface. Place the tube over the animal’s head, and immediately tap the rump to prevent the animal from backing up. Once it is in the device, slide the plastic gate into the appropriate slot to secure the animal’s position.
The third type of device that we’ll discuss is tube restraints. These are Plexiglass cylinders with one closed end containing holes for air circulation, and a slit at the open end. To use a tube restraint, grasp the animal and place it on a smooth surface. Next, place the open end of the tube at the animal’s head at a 45° angle. As soon as the rodent enters the tube, tap the rump to prevent it from backing up. Finally, occlude the opening by stuffing a large latex glove, which will grip the Plexiglass surface and prevent the animal from exiting the tube.
Lastly, we will discuss how to use flexible restrainers, which are disposable flexible plastic cones available in different sizes. Selection of a cone depends on the animal size. Ideally, it should be long enough to extend 2-3 inches beyond the animal’s rump. Like previous methods, place the animal on a smooth surface and place restraint cone at a 45° angle over the animal’s head. As soon as the animal enters the cone, grasp the open end and seal it to force the animal to the end. Secure the opening by folding the plastic to one side of the tail and applying a binder clip to the folded plastic being careful to avoid clamping the tail or skin.
After discussing the basics of handling and different restraining techniques, let’s review why having an understanding of these procedures is necessary for any in vivo scientist.
The knowledge and rationale behind these basic handling procedures is at the core of maintaining animal’s health. From the time the animals are born, they are handled for simple, day-to-day activities like weighing, inspection and cage changing, which ought to be performed irrespective of the scientific objective.
The restraining procedures are also useful for marking purposes. For example, in order to perform procedures like ear punching the animal need to be restrained by scruffing. On the other hand, a restraint device should be used when inserting a microchip in rats so that they can be tagged permanently.
Lastly, the manual restraining methods are used for common injection procedures like subcutaneous and intraperitoneal administration. And the knowledge of restrain devices is useful when performing intravenous administration, which demands minimal animal movement during the injection.
You’ve just watched JoVE’s introduction to basic handling and restraint techniques for rodents. These procedures require a confident and firm, but gentle touch. Tentative approaches can result in handler bites, while aggressive handling can cause injury to the rodent. Therefore, it is important to learn these techniques thoroughly and find a balance of an assertive yet gentle grip. As always, thanks for watching!
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