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siRNA Screening naar Ubiquitin en Ubiquitin-achtig systeem regulatoren van biologische pathways identificeren in gekweekte zoogdiercellen
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siRNA Screening naar Ubiquitin en Ubiquitin-achtig systeem regulatoren van biologische pathways identificeren in gekweekte zoogdiercellen

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

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Hier beschrijven we een methode om een ​​gerichte siRNA "ubiquitome" scherm om nieuwe ubiquitine en ubiquitine-achtige regulatoren van de-HIF1a gemedieerde cellulaire respons op hypoxie identificeren voeren. Dit kan worden aangepast aan elke biologische weg wanneer een robuuste uitlezing van reporter-activiteit beschikbaar.

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Biochemie siRNA screening ubiquitine UBL ubiquitome hypoxie HIF1a High throughput zoogdiercellen luciferase reporter
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