Fluorescence and Bioluminescence Imaging of Subcellular Ca²⁺ in Aged Hippocampal Neurons

7.9K צפיות

צוטט 6 פעמים

13:24 דק׳

1 בדצמבר 2015

10.3791/53330-v

1 בדצמבר 2015

7.9K צפיות

María Calvo-Rodríguez¹ , Carlos Villalobos¹ , Lucía Nuñez²

¹Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, ²Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid

Intracellular Ca²⁺ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca²⁺ overload. We aimed at investigating Ca²⁺ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca²⁺ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.