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JoVE Journal Bioengineering
Migliorare 2D e 3D della pelle
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Migliorare 2D e 3D della pelle

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Vi presentiamo un protocollo per ottenere matrici derivati ​​dalle cellule ricche di proteine ​​della matrice extracellulare, utilizzando Crowders macromolecolari (MMC). Inoltre, vi presentiamo un protocollo che incorpora MMC nella generazione di pelle co-coltura organotipica 3D, che riduce i tempi di cultura, pur mantenendo la maturità di costrutto.

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Bioingegneria cheratinociti i fibroblasti affollamento macromolecolare coltura cellulare 2D coltura delle cellule 3D co-colture organotipiche pelle matrice extracellulare collagene di tipo I collagene di tipo IV collagene di tipo VII, giunzione dermo-epidermica
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