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JoVE Journal Chemistry
Gelijktijdige meting van HDAC1 en HDAC6 activiteit in HeLa cellen met behulp van UHPLC-MS
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Gelijktijdige meting van HDAC1 en HDAC6 activiteit in HeLa cellen met behulp van UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

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De huidige methode dient ter identificatie van isovorm-specifieke remmers van Histon deacetylases (HDAC) in HeLa cellen door de UHPLC-MS-analyse van meerdere substraten. Dit is een antilichaam-vrije methode ontwikkeld om te reflecteren van HDAC1 en HDAC6 activiteit in de levende cel omgeving, in tegenstelling tot één-isovorm cel-gratis testen.

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Chemie kwestie 126 HDAC1 HDAC6 remmers cel-gebaseerde assay UHPLC-MS HeLa cellen
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