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JoVE Journal Cancer Research
GFP レンチ ウイルス導入オルガノイド培養患者由来大腸腫瘍によって生成される微小の高感度検出
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
Journal (Cancer Research)
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
Journal (Cancer Research)
Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
 
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GFP レンチ ウイルス導入オルガノイド培養患者由来大腸腫瘍によって生成される微小の高感度検出

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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总结概括
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CRC の PDX 由来器官毛細細胞に緑色蛍光タンパク質 (GFP) レンチ ウイルス粒子の効率的な伝達のためのプロトコルを示す本普及のひと大腸癌の高感度検出 (CRC) 細胞組織を植民地化をできるように、ステレオ蛍光顕微鏡による観察で、受信者のマウスに注入する前に。

Tags

がん研究、問題 136、人間の CRC、患者由来腫瘍異種移植 (PDXs)、PDX 由来腫瘍の GFP 標識 organoids、レンチ ウイルス伝達、微小転移、3次元培養
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