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Journal / Immunology and Infection / Generasjon av Knock-out primære og utvidet…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generasjon av Knock-out primære og utvidet menneskelige NK celler ved hjelp av Cas9 Ribonucleoproteins
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Generasjon av Knock-out primære og utvidet menneskelige NK celler ved hjelp av Cas9 Ribonucleoproteins

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Her presenterer vi en protokoll for genetisk endre primær eller utvidet menneskets naturlige killer (NK) celler ved hjelp av Cas9 Ribonucleoproteins (Cas9/RNPs). Bruker denne protokollen, generert vi menneskelige NK celler mangelfull for omforming vekst faktor-b reseptor 2 (TGFBR2) og hypoxanthine phosphoribosyltransferase 1 (HPRT1).

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Immunologi og infeksjon problemet 136 menneskelige primære NK celler menneskelige utvidet NK celler CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ motstandsdyktig NK celler
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