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Journal / Immunology and Infection / Digestão do fígado murino para um fluxo…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestão do fígado murino para um fluxo Cytometric análise de células endoteliais linfáticas
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Digestão do fígado murino para um fluxo Cytometric análise de células endoteliais linfáticas

Article DOI: 10.3791/58621-v • 08:07 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1,2
1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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O objetivo do presente protocolo é identificar populações de células endoteliais linfáticas dentro do fígado usando marcadores descritos. Nós utilizamos colagenase IV e DNase e um suave de picagem de tecido, combinado com citometria de fluxo, para identificar uma população distinta de células endoteliais linfáticas.

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Imunologia e infecção edição 143 fígado células endoteliais linfáticas citometria de fluxo fígadas células endoteliais sinusoidais colagenase IV
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