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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse af Shear Flow-induceret Migration af Murine Marginal Zone B-celler In Vitro
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Analyse af Shear Flow-induceret Migration af Murine Marginal Zone B-celler In Vitro

Article DOI: 10.3791/58759-v • 08:31 min • November 26th, 2018
November 26th, 2018 •
1, 1, 1, 1, 1
1Institute of Biochemistry and Cell Biology, OVGU University of Magdeburg

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Marginal zone B celler (MZBs) reagere på kraften af shear flow ved re orientere deres overflytningssti op for strømmen. Denne protokol viser, hvordan man registrere og analysere migration ved hjælp af en fluidics enhed, pumpe, mikroskop billedbehandlingssystem og fri software.

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Immunologi og infektion sag 141 B-celler marginale zone migration shear flow integriner vedhæftning intercellulære vedhæftning molekyle-1 (ICAM-1) vaskulære celle vedhæftning molekyle-1 (VCAM-1)
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