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Extracellular 소포 세포 조절 미디어에서의 Cytometric 분석 흐름

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

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프로토콜 셀 문화 supernatants 함께 사용 하기 위해 작은 extracellular 소포 (EV)에 표면 epitopes를 감지 하도록 설계 된 재현 방법을 설명 합니다. 그것은 항 체를 표면 항 원 CD9, 인식 CD81 그리고 CD63, 함께 하는 비즈를 사용 하 여 특정 EV immunoprecipitation 활용. 메서드는 다운스트림 흐름 cytometry 분석에 최적화 되어 있습니다.

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생물학 문제 144 exosomes microvesicles cytometry exosomes 형질 구슬 바른된 중간 extracellular 소포
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