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Immunology and Infection

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Isolamento de células mononucleares de lamina propria de cólon murino usando Collagenase E
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Isolamento de células mononucleares de lamina propria de cólon murino usando Collagenase E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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O objetivo deste protocolo é isolar as células mononucleares que residem na lâmina própria do cólon por digestão enzimática do tecido usando Collagenase. Este protocolo permite a isolação eficiente de pilhas mononucleares tendo por resultado uma única suspensão da pilha que por sua vez possa ser usada para immunophenotyping robusto.

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Imunologia e infecção linfócitos cólon murino lamina propria colite transplante citometria de fluxo digestão enzimática inflamação células mononucleares
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