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Immunology and Infection

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Intravital Imaging af Intraepitelial lymfocytter i murine tyndtarmen

Article DOI: 10.3791/59853-v • 08:00 min • June 24th, 2019
June 24th, 2019 •
1, 1
1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School

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Vi beskriver en metode til at visualisere GFP-mærket γδ IELs ved hjælp af intravital billeddannelse af murine tyndtarmen ved inverteret spinning disk confokal mikroskopi. Denne teknik gør det muligt at spore levende celler i slimhinden i op til 4 timer og kan bruges til at undersøge en række af intestinal immun-epitheliale interaktioner.

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Immunologi og infektion immunologi T-celle intraepitelial lymfocytter mus tyndtarm intravital billeddannelse spinning disk refleksionskonfokalmikroskopi mikroskopi celle tracking
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