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Het verkrijgen van primaire osteocyten door murine calvariale fractionering van GFP-tot expressie brengende osteocyten
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Het verkrijgen van primaire osteocyten door murine calvariale fractionering van GFP-tot expressie brengende osteocyten

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Dit protocol beschrijft de dissectie van neonatale dmp1-topaas muizen calvaria en isolatie van osteocyten die het groene fluorescerende eiwit tot expressie brengen door celvertering en fractionering, naast osteocytenpreparaat voor fluorescentie geactiveerde celsortering (FACS).

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Retractie Primaire osteocyt Dmp1 SOST GFP Fractionering FACS
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