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Geração e Expansão de Cardiomiócitos Humanos a partir de células mononucleares de sangue periféricos do paciente

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Aqui, apresentamos um protocolo para gerar e expandir robustamente os cardiomiócitos humanos a partir de células mononucleares de sangue periféricos do paciente.

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Biologia do Desenvolvimento Questão 168 células mononucleares periféricas PBMCs células-tronco pluripotentes induzidas pelo homem iPSCs diferenciação cardíaca reprogramação do iPSC expansão do cardiomiócito.
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