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Immunology and Infection

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JoVE Journal Immunology and Infection
Adaptação de organoides gastrointestinais para infecção por patógenos e sequenciamento de células únicas sob condições de nível de biossegurança 3 (BSL-3)
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Adaptação de organoides gastrointestinais para infecção por patógenos e sequenciamento de células únicas sob condições de nível de biossegurança 3 (BSL-3)

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

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Este protocolo descreve como infectar organoides intestinais humanos de seu lado apical ou basolateral para caracterizar interações hospedeiro/patógeno no nível de célula única usando a tecnologia de sequenciamento de RNA unicelular (scRNAseq).

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Imunologia e Infecção Problema 175 organoides intestinais humanos sequenciamento de RNA unicelular nível de biossegurança 3 infecção organoide patógenos entericos infecção apical e basolateral
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