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Journal / Immunology and Infection / Генерация трансгенов и нокаутов у видов…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Генерация трансгенов и нокаутов у видов стронгилоидов путем микроинъекции
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Генерация трансгенов и нокаутов у видов стронгилоидов путем микроинъекции

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Функциональный геномный инструментарий для паразитических нематод Strongyloides stercoralis и Strongyloides ratti включает трансгенез, CRISPR/Cas9-опосредованный мутагенез и RNAi. Этот протокол продемонстрирует, как использовать внутригонадную микроинъекцию для введения трансгенов и компонентов CRISPR в S. stercoralis и S. ratti.

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