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JoVE Journal Developmental Biology
Differentiering af humane pluripotente stamceller til pancreas betacelleprækursorer i et 2D-kultursystem
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Differentiering af humane pluripotente stamceller til pancreas betacelleprækursorer i et 2D-kultursystem

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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Den nuværende protokol beskriver en forbedret metode til at øge co-ekspressionen af PDX1 og NKX6.1 transkriptionsfaktorer i bugspytkirtlen forfædre afledt af humane pluripotente stamceller (hPSC'er) i plane monolag. Dette opnås ved at genopfylde den friske matrix, manipulere celletæthed og dissociere de endodermale celler.

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Udviklingsbiologi udgave 178
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