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Concanavalin A-basert sedimenteringsanalyse for å måle substratbinding av glukanfosfataser
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Concanavalin A-basert sedimenteringsanalyse for å måle substratbinding av glukanfosfataser

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Denne metoden beskriver en lektinbasert in vitro sedimenteringsanalyse for å kvantifisere bindingsaffiniteten til glukanfosfatase og amylopektin. Denne samsedimenteringsanalysen er pålitelig for måling av glukanfosfatasesubstratbinding og kan påføres forskjellige løselige glukansubstrater.

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