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Edição de Base Eficiente sem PAM para Modelagem de Zebrafish de Doenças Genéticas Humanas com zSpRY-ABE8e
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Edição de Base Eficiente sem PAM para Modelagem de Zebrafish de Doenças Genéticas Humanas com zSpRY-ABE8e

Article DOI: 10.3791/64977-v • 07:31 min • February 17th, 2023
February 17th, 2023 •
1,2, 3, 1,2, 4, 1,2, 1,2
1Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, 2Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 3Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, 4Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences

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Este protocolo descreve como realizar uma edição eficiente da base de adenina sem limitação de PAM para construir um modelo preciso de doença do peixe-zebra usando zSpRY-ABE8e.

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