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JoVE Journal Medicine
Isolatie van menselijke sapheneuze ader-endotheelcellen en blootstelling aan gecontroleerde niveaus van schuifspanning en rek
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Isolatie van menselijke sapheneuze ader-endotheelcellen en blootstelling aan gecontroleerde niveaus van schuifspanning en rek

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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We beschrijven een protocol om menselijke venaeuze veneuze endotheelcellen (hSVECs) te isoleren en te kweken. We bieden ook gedetailleerde methoden om schuifspanning en rek te produceren om mechanische spanning in hSVECs te bestuderen.

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Deze maand in JoVE nummer 194
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