Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay

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Zinc transport has proven challenging to measure due to the weak causal links to protein function and the low temporal resolution. This protocol describes a method for monitoring, with high temporal resolution, Zn²⁺ extrusion from living cells by utilizing a Zn²⁺ sensitive fluorescent dye, thus providing a direct measure of Zn²⁺ efflux.