Zhejiang University of Technology 5 articles published in JoVE Environment Elucidating the Metabolism of 2,4-Dibromophenol in Plants Juan Wu1, Xindong Yang2, Qianwen Wang3, Qinghua Zhou1, Anping Zhang2, Jianqiang Sun1 1Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, 2International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), College of Environment, Zhejiang University of Technology, 3Research and Teaching Center of Agriculture, Zhejiang Open University The present protocol describes a simple and efficient method for the identification of 2,4-dibromophenol metabolites in plants. Biochemistry A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro Xinwen Miao*1, Jiawei Wu*1,2, Wenting Zhao1,3 1School of Chemistry, Chemical Engineering, and Biotechnology, Nanyang Technological University, 2State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, 3Institute for Digital Molecular Analytics and Science, Nanyang Technological University Here, a nanobar-supported lipid bilayer system is developed to provide a synthetic membrane with a defined curvature that enables the characterization of proteins with curvature sensing ability in vitro. Environment Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell Liyi Wang1,2, Nana Xue1,2, Wenfeng Li2, Rehemanjiang Wufuer1, Daoyong Zhang1,3 1Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology This paper introduces a method of hatching without using an eggshell for toxicological studies of particle pollutants such as microplastics. Environment Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish Chao Xu1, Hangqin Guo1, Ruijiong Wang1, Tianyang Li1, Linqi Gu1, Liwei Sun1 1College of Environment, Zhejiang University of Technology Zebrafish embryos/larvae develop externally and are optically transparent. The bioaccumulation of microplastics in fish at early life stages is readily assessed with fluorescently labeled microbeads. Engineering CO2 Photoreduction to CH4 Performance Under Concentrating Solar Light XiaoXiang Fang1, Zhihong Gao1, Hanfeng Lu1, Qiulian Zhu1, Zekai Zhang1 1Institute of Chemical Reaction Engineering, College of Chemical Engineering, Zhejiang University of Technology We present a protocol for improving the performance of CO2 photoreduction to CH4 by heightening the incident light intensity via concentrating solar energy technology.