3B BlackBio Biotech India Limited 1 article published in JoVE Biology Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA Satyabrata Bag1, Santanu Ghosh1, Roopa Lalwani1, Pratishtha Vishnoi1, Sudheer Gupta1, Dhirendra Dubey1 13B BlackBio Biotech India Limited Real-time polymerase chain reaction (PCR)-based detection and quantification of hepatitis B virus (HBV) DNA is a sensitive and accurate method for diagnosing and monitoring HBV infection. Here, we present a protocol for HBV DNA detection and the viral load measurement of a sample.