Curtin University of Technology View Institution's Website 1 article published in JoVE Biology Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction Avdesh Avdesh*1,2, Mengqi Chen*1,3, Mathew T. Martin-Iverson1,2,4, Alinda Mondal1,3, Daniel Ong1, Stephanie Rainey-Smith1,3, Kevin Taddei1,3, Michael Lardelli5, David M. Groth6, Giuseppe Verdile1,3, Ralph N. Martins1,2,3,7 1Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, 2Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, 3McCusker Alzheimer's Research foundation, 4School of Medicine and Pharmacology, University of Western Australia, 5Department of Molecular and Biomedical Sciences, University of Adelaide, 6School of Biomedical Sciences, Curtin University of Technology, 7School of Psychiatry and Clinical Neurosciences, University of Western Australia This protocol outlines regular maintenance and care to maintain optimal conditions for zebrafish husbandry. The video illustrates the protocol for system maintenance, regular housing, feeding, breeding, and raising of zebrafish larvae.