Universitätsklinikum Essen View Institution's Website 1 article published in JoVE Neuroscience Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells Kristian Pajtler*1, Anna Bohrer*1, Jochen Maurer2, Hubert Schorle2, Alexander Schramm1, Angelika Eggert1, Johannes Hubertus Schulte1 1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.