Odense University Hospital 5 articles published in JoVE Medicine Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy Sabrina T. Hansen1,2, Charlotte H. Jensen2,3,4, Jens A. Sørensen2,4,5, Søren P. Sheikh2,3,4, Lars Lund1,2,4 1Department of Urology, Odense University Hospital, 2Clinical Institute, University of Southern Denmark, 3Laboratory of Molecular and Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, 4Center for Vascular Regeneration, Odense University Hospital, 5Research Unit for Plastic Surgery, Odense University Hospital Precise disclosure of methods and protocols is crucial for large scale uptake of stem cell therapies. Here, we present a protocol to isolate adipose-derived regenerative cells, used for a single intracavernous injection as treatment of erectile dysfunction (ED) following radical prostatectomy (RP). Medicine A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice Alexander Wiinholt1,2, Mads G. Jørgensen2, Amar Bučan1,2, Farima Dalaei1,2, Jens A. Sørensen2 1University of Southern Denmark, 2Department of Plastic Surgery, Odense University Hospital This animal model enables researchers to induce statistically significant secondary lymphedema in the hindlimb of mice, lasting at least 8 weeks. The model can be used to study the pathophysiology of lymphedema and to investigate novel treatment options. Immunology and Infection Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes Jeff Granhøj1, Henrik Dimke1,2, Per Svenningsen1 1Department of Molecular Medicine, University of Southern Denmark, 2Department of Nephrology, Odense University Hospital Here we present a method to select for novel variants of the E. coli biotin-protein ligase BirA that biotinylates a specific target peptide. The protocol describes the construction of a plasmid for the bacterial display of the target peptide, generation of a BirA library, selection and characterization of BirA variants. Medicine Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat Morten Hox1, Travis Mann-Gow2, Lars Lund3,4, Peter Zvara1,4 1Biomedical Laboratory and the Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, 2Department of Surgery, University of Vermont, 3Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, 4Department of Urology, Odense University Hospital This study describes a simplified surgical procedure and technique for performing cavernous nerve stimulation with the isolation of the nerve-electrode complex using silicone glue and intracavernous pressure measurement. Bioengineering Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy Maria Bloksgaard1, Bjarne Thorsted2, Jonathan R. Brewer2, Jo G. R. De Mey1,3 1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.