Ege University 1 article published in JoVE Biochemistry Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein Alev Tascioglu Aliyev1,2, Francesca LoBianco1, Kimberly J. Krager1, Nukhet Aykin-Burns1 1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.