Amnis Corporation View Institution's Website 1 article published in JoVE Bioengineering Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry Yashdeep Phanse1, Amanda E. Ramer-Tait1, Sherree L. Friend2, Brenda Carrillo-Conde3, Paul Lueth1, Carrie J. Oster1, Gregory J. Phillips1, Balaji Narasimhan3, Michael J. Wannemuehler1, Bryan H. Bellaire1 1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.