Name: centrosome duplication in cell division
Uploaded: 2023-04-30T18:28:50.000Z
Duration: 2 min 25 s
Channel: JoVE
Description: Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Centrosome Duplication

Ein Zentrosom ist der Hauptorganisator der Mikrotubuli einer eukaryotischen Zelle und spielt eine wichtige Rolle bei der Bildung der mitotischen Spindel, einer bipolaren Anordnung von Mikrotubuli, die die Schwesterchromatiden trennt. Die beiden Spindelpole werden durch zwei gegeneinander angeordnete Zentrosomen gebildet.
Vor dem Zellzyklus enthalten die meisten tierischen Zellen jedoch nur ein Zentrosom im Zytoplasma. Die G1/S-Cyclin-abhängige Kinase oder Cdk-Komplex, die in tierischen Zellen aus Cyclin E und Cdk2 besteht, trägt dazu bei, die Zentrosomduplikation sowie den Eintritt in den Zellzyklus auszulösen. 
Jedes Zentrosom besteht aus zwei Zentriolen, die von einer proteinhaltigen perizentriolären Matrix verkapselt sind.
Die Zentrosomduplikation beginnt in der frühen G1-Phase, wenn die Separase die Entkopplung der beiden Zentriolen durchführt. Plk-4, ein Protein aus der Familie der Polo-ähnlichen Proteinkinasen, ist ein wichtiger Regulator für die Zentriol-Biogenese beim Menschen.
Zu Beginn der S-Phase beginnt ein Tochterzentrriol im rechten Winkel zur Basis jeder Zentriole zu wachsen.
Während G2 beenden die Töchterzentriolen typischerweise die Verlängerung. Die vier Zentriolen bleiben im Zentrosom dicht beieinander, bis die Zelle in die Mitose eintritt.
Zu Beginn der Mitose teilt sich das Zentrosom in zwei Teile, was auch als Zentrosomdisjunktion bekannt ist. Die beiden resultierenden Zentrosomen enthalten jeweils ein Paar Zentriolen und die perizentrioläre Matrix. Beide Zentrosomen keimen einen Satz von Mikrotubuli, hauptsächlich an der Mutterzentriole, und bilden zusammen die mitotische Spindel.
Der Lebenszyklus von Zentrosom und Chromosom ist in mehreren Aspekten ähnlich. Ihre Duplizierung erfolgt nur einmal pro Zyklus und beide duplizieren sich durch einen semikonservativen Prozess, bei dem eine neue Kopie aus der bereits vorhandenen übergeordneten Kopie erstellt wird.
Wie bei den Chromosomen wird auch eine Kopie des Zentrosoms nach der Teilung gleichmäßig auf jede Tochterzelle verteilt.
Eine übermäßige Zentrosomduplikation kann die mitotische Spindelassemblierung beeinträchtigen und dazu führen, dass sich die Chromosomen nicht richtig trennen, was zu Krankheiten wie Krebs führen kann.

<ol>
	<li style="margin-left: 0.5in;">Fujita, Hiroki, Yuki Yoshino und Natsuko Chiba. 2015. "Regulation des Zentrosomenzyklus." Molekulare und zelluläre Onkologie 3 (2). https://doi.org/10.1080/23723556.2015.1075643.</li>
	<li style="margin-left: 0.5in;">Nigg, Erich A. und Andrew J. Holland. 2018. "Einmal und nur einmal: Mechanismen der Zentriol-Duplikation und ihre Deregulierung bei Krankheiten." Nature Reviews Molecular Cell Biology 19 (5): 297–312. https://doi.org/10.1038/nrm.2017.127.</li>
</ol>

zentrosomen-duplikation

Das primäre mikrotubuläre Organisationszentrum (MTOC) in tierischen Zellen ist das Zentrosom. Ein Zentrosom besteht im Kern aus zwei zylindrischen Zentriolen. Jede Zentriole besteht aus neun Sätzen von drei Mikrotubuli, die durch Proteine zusammengehalten werden. Die Zentriolen sind rechtwinklig zueinander positioniert und von einer formlosen Proteinwolke umgeben, die als perizentrioläre Matrix oder perizentrioläres Material (PCM) bezeichnet wird.

Um sicherzustellen, dass jede Tochterzelle nach der Zellteilung ein Zentrosom erhält, beginnt die Verdopplung des Zentrosoms früh im Zellzyklus. Die Verdopplung des Zentrosoms wird streng durch Zellzykluskontrollen - wie die zyklinabhängige Kinase 2 (Cdk2)- reguliert, um zu verhindern, dass sie mehr als einmal pro Zellzyklus stattfindet. Wenn die Zelle die Mitose erreicht, hat sie daher zwei Zentrosomen.

Die Verdopplung des Zentrosoms fällt mit den Phasen des Zellzyklus zusammen. Während der G1-Phase des Zellzyklus trennen sich die beiden Zentriolen im Zentrosom, ein Prozess, der als Zentrosom-Desorientierung bezeichnet wird.

Während der G1- und S-Phasen werden die Zentrosomen verdoppelt. Ein neues Zentriol, das als Prozentiol bezeichnet wird, beginnt sich an der Basis jedes der beiden vorhandenen Zentriolen zu bilden und zu verlängern. Die Prozentiolen verlängern sich während der S- und G2-Phasen, bis sie so groß wie die älteren Zentriolen sind. Die vier Zentriolen bleiben innerhalb der vergrößerten PCM bis zum Eintritt der Zelle in die Mitose eng zusammen.

Während der G2-Phase reichern sich γ-Tubulin und andere PCM-Proteine im Zentrosom an, ein Prozess, der als Zentrosomenreifung bezeichnet wird.

Während des Übergangs von der G2- zur M-Phase beginnen sich die Zentrosomen zu trennen. Die beiden Mutterzentriolen werden getrennt, und mikrotubuläre Motorproteine bewegen die beiden Zentrosomen auseinander.

Fehler in der Regulation der Zentrosomen können zu Anomalien in der Anzahl der Chromosomen und Zentrosomen führen. Zentrosomenanomalien und Defekte in der Progression des Zentrosomenzyklus sind in mehreren Krankheiten, insbesondere in Krebs, impliziert. Tumorsuppressorproteine und Onkogene sind mit nachteiligen Veränderungen in den Zentrosomen von Tumorzellen verknüpft, was diese Proteine zu einem attraktiven Behandlungsziel macht.

Zentrosomen-Duplikation

Das primäre Mikrotubuli-Organisationszentrum (MTOC) in tierischen Zellen ist das Zentrosom. Ein Zentrosom hat in seinem Kern zwei zylindrische Zentriolen. Jeder...

Education

Lehre

JoVE Core

Cell Biology

Zellbiologie

Unit 1

Einheit 1

Cell Division

Zellteilung

KEY TERMS AND CONCEPTS

KERNBEGRIFFE UND KONZEPTE

centrosome duplication in cell division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

Centrosome Duplication in Cell Division

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

centrosome duplication

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Chromatid Pulling in Cell Division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

Spindle Position and Cell Division Plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic Content During Cell Division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how centrosome duplication supports cell division and helps a cell prepare for the next stage of growth and division in grades 9–12 biology.

Centrosome Duplication in Cell Division | JoVE K12

cell biology

JoVE Core Zellbiologie veranschaulicht komplexe zelluläre Prozesse in leicht verständlichen und dennoch umfassenden animierten Videolektionen. Diese Sammlung konzentriert sich auf die wesentlichen Bestandteile und Funktionen von Zellen, einschließlich Organellen und Membranen, Zelltransport und Zellenergie. Ergänzt wird sie durch Videos von Wissenschaftlern in Aktion, die die Anwendung der Zellbiologie in realen Forschungsexperimenten demonstrieren.

Louisiana Student Standards for Science

High School

Life Sciences

from-molecules-to-organisms-structures-and-processes

From Molecules To Organisms: Structures And Processes

HS-LS1-4

Tennessee Academic Standards

Biology

From Molecules to Organisms: Structures and Processes

BIO1.LS1.6

Minnesota Academic Standards in Science

3.1.1 9L.3.1.1.2

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

Arizona Science Standards

HS+B.L1

HS+B.L1U1.9

Alabama Course of Study Science

9-12.B.1.3

Next Generation Science Standards

Florida Department of Education

SC.912.L.16

SC.912.L.16.14

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

B-LS1-4.

new-york-state-science-learning-standards

New York State Science Learning Standards

HS-LS1-4.

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3.1.9-12.P

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3.1.9-12.Q

Centrosome Duplication in Cell Division

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