Name: spindle position and cell division plane
Uploaded: 2023-04-30T18:28:51.000Z
Duration: 2 min 13 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Determining the Plane of Cell Division

Eine sich teilende Zelle, die ihre Zellteilungsebene genau bestimmt, ist essentiell für die erfolgreiche Trennung von Chromosomen und die Verteilung des zytoplasmatischen Inhalts an ihre Tochterzellen.
Bei Tieren befindet sich die Teilungsebene am Zelläquator zwischen den segregierten Chromosomen, und ihre Lage wird während der Anaphase bestimmt. Mikrotubuli, die die mitotische Spindel bilden, interagieren mit der Zellrinde und spielen eine entscheidende Rolle bei der Spezifizierung der Spaltfurche.
Drei verschiedene Modelle werden verwendet, um die Bestimmung der Zellteilungsebene zu erklären – das astrale Stimulationsmodell, das polare Relaxationsmodell und das Modell der zentralen Spindelstimulation.
Im astralen Stimulationsmodell interagieren radiale Anordnungen astraler Mikrotubuli mit dem äquatorialen Kortex und induzieren die Bildung der Spaltfurche.
Im polaren Relaxationsmodell senden astrale Mikrotubuli hemmende Signale an die Zellpole und unterdrücken so die Bildung der Spaltfurche an beiden Polen. Dies führt zu einer kortikalen Kontraktion am Zelläquator.
Sobald die Anaphase beginnt, ordnen sich die interpolaren Mikrotubuli, die zwischen den segregierten Chromosomen vorhanden sind, in den überlappenden antiparallelen Mikrotubuli an, die auch als zentrale Spindel bezeichnet werden. Im Modell der Zentralspindelstimulation stimuliert die Zentralspindel die Bildung von Spaltfurchen am äquatorialen Kortex. 
Der allgemeine Konsens ist, dass alle drei Prozesse zusammen wirken, um die Ebene der Zellteilung zu bestimmen. Dieser mehrgleisige Ansatz kann dazu führen, dass das System auf Schwankungen der Proteinkonzentration sowie der Zellgröße reagiert und dadurch die Genauigkeit der Zellteilung erhöht.
Der Beitrag jeder Methode hängt vom Zelltyp und der Zellgröße ab. Zum Beispiel ist in C. elegans-Embryonen ein großes Array von astralen Mikrotubuli vorhanden und könnte daher eine aktivere Rolle bei der Bestimmung der Zellteilungsebene spielen.

<ol>
	<li>Molekularbiologie der Zelle, Alberts, 6. Auflage. Seiten 997-999.</li>
	<li>Molekulare Zellbiologie, Lodish, 8. Auflage. Seiten - 903.</li>
	<li>Geelen, Danny. "Die Positionierung der Teilungsebene wird durch eine kortikale Teilungsstelle an der Plasmamembran bestimmt." In Biotechnologie und nachhaltige Landwirtschaft 2006 und darüber hinaus, S. 121-124. Springer, Dordrecht, 2007.</li>
	<li>Balasubramanian, Mohan K., Erfei Bi und Michael Glotzer. "Vergleichende Analyse der Zytokinese in knospenden Hefen, Spalthefen und tierischen Zellen." Current Biology 14, Nr. 18 (2004): R806-R818.</li>
	<li>Chant, John. "Septin-Gerüste und Spaltebenen in Saccharomyces." Zelle 84, Nr. 2 (1996): 187-190.</li>
	<li>Rincon, Sergio A. und Anne Paoletti. "Mid1/Anillin und die räumliche Regulation der Zytokinese in der Spalthefe." Zytoskelett 69, Nr. 10 (2012): 764-777.</li>
	<li>Galli, Matilde und Sander van den Heuvel. "Bestimmung der Spaltebene in frühen C. elegans-Embryonen." Jahresbericht der Genetik 42 (2008): 389-411.</li>
</ol>

bestimmung der ebene der zellteilung

Die Positionierung der Zellteilungsebene ist ein kritischer Schritt während der Entwicklung und Zelldifferenzierung, insbesondere während der Mitose, wenn die Ebene entscheidend für die Größe der beiden Tochterzellen ist. Die Zellteilungsebene steht senkrecht zur Ebene der Chromosomensegregation, jedoch haben verschiedene Arten von Organismen unterschiedliche Mechanismen der Zellteilung, die ihrer Morphologie und Funktion entsprechen.

Tierische Zellen

In tierischen Zellen bildet sich die Schnürfurche entlang der Zellteilungsebene, beginnend von der Zellrinde, dem Bereich unterhalb der Plasmamembran. Der mitotische Spindelapparat spielt eine entscheidende Rolle bei der Bestimmung der Zellteilungsebene; daher kann eine Änderung der Achse des mitotischen Spindels die Teilungsebene verändern. Diese Strategie wird von einigen eukaryotischen Organismen wie Caenorhabditis elegans genutzt, um von symmetrischer zu asymmetrischer Zellteilung zu wechseln. Symmetrische Zellteilung produziert zwei identische Tochterzellen und wird oft verwendet, um Gewebe zu wachsen, das viele gleichartige Zellen enthält. Im Gegensatz dazu schafft die asymmetrische Teilung zelluläre Vielfalt, da sie zwei nicht identische Tochterzellen erzeugt. Zum Beispiel vermehren Neuroepithelzellen zunächst ihre Anzahl durch symmetrische Teilungen. Danach nutzen sie asymmetrische Teilung, um Tochterzellen zu produzieren, die der Mutterzelle ähnlich sind sowie differenzierende Neuronen.

Hefe

In Sprosshefe, wie Saccharomyces cerevisiae, wird die Ebene der Zellteilung in der G1-Phase durch Septin-Proteine bestimmt. Die Zytokinese wird durch die Bildung einer Schnürfurche im Halsbereich der Sprosshefezellen erreicht; jedoch spielt der mitotische Apparat keine Rolle bei der Bestimmung der Zellteilungsebene. Im Gegensatz dazu entscheidet in der Spalthefe, wie Schizosaccharomyces pombe, die Position des Interphasekerns in der G2-Phase über den Ort der Zellteilungsebene. Während der Interphase ist das Mid1-Protein hauptsächlich im Kern lokalisiert; jedoch wird es während der Mitose aus dem Kern exportiert und rekrutiert im Zellkortex Komponenten des Aktomyosinrings in den mittleren Bereich der Zelle. Dies führt letztlich dazu, dass sich die Mutterzelle in zwei gleich große Tochterzellen teilt.

Pflanzen

In den meisten Pflanzenzellen wird die Ebene der Zellteilung vor dem Eintritt in die Mitose festgelegt. Ein Präprophaseband bildet sich entlang des Äquators der Elternzelle, das die Zellteilungsebene markiert. Das Präprophaseband ist eine ringförmige Struktur aus Mikrotubuli und F-Aktin. Es gibt Anlass zu einem Mikrotubuli-Bündel namens Phragmoplast, das ein strukturelles Gerüst für die Expansion und Positionierung der Zellplatte bietet.

Bestimmung der Ebene der Zellteilung

Die Positionierung der Zellteilungsebene ist ein kritischer Schritt während der Entwicklung und Zelldifferenzierung, insbesondere während der Mitose, wenn die Ebene ...

Education

Lehre

JoVE Core

Cell Biology

Zellbiologie

Unit 1

Einheit 1

Cell Division

Zellteilung

KEY TERMS AND CONCEPTS

KERNBEGRIFFE UND KONZEPTE

spindle position and cell division plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division.

This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form.

The transcript focuses on how the spindle is used to determine that plane. By tracking spindle position, scientists can study how division is oriented inside the cell. This makes the concept easier to connect to the steps of mitosis and cell structure.

Spindle Position and Cell Division Plane

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

centrosome duplication

Centrosome Duplication in Cell Division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Chromatid Pulling in Cell Division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic Content During Cell Division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how spindle position helps determine the plane of cell division during mitosis and why this matters for daughter cell arrangement in developing tissues.

Spindle Position and Cell Division Plane | JoVE K12

cell biology

JoVE Core Zellbiologie veranschaulicht komplexe zelluläre Prozesse in leicht verständlichen und dennoch umfassenden animierten Videolektionen. Diese Sammlung konzentriert sich auf die wesentlichen Bestandteile und Funktionen von Zellen, einschließlich Organellen und Membranen, Zelltransport und Zellenergie. Ergänzt wird sie durch Videos von Wissenschaftlern in Aktion, die die Anwendung der Zellbiologie in realen Forschungsexperimenten demonstrieren.

Louisiana Student Standards for Science

High School

Life Sciences

from-molecules-to-organisms-structures-and-processes

From Molecules To Organisms: Structures And Processes

HS-LS1-4

Tennessee Academic Standards

Biology

From Molecules to Organisms: Structures and Processes

BIO1.LS1.6

Minnesota Academic Standards in Science

3.1.1 9L.3.1.1.2

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

Arizona Science Standards

HS+B.L1

HS+B.L1U1.9

Alabama Course of Study Science

9-12.B.1.3

Florida Department of Education

SC.912.L.16

SC.912.L.16.14

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

B-LS1-4.

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell&#8217;s life. In eukaryotic cells, the somatic cell cycle has two ...

Interphase

The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the ...

The Cell Cycle Control System

Positive Regulator Molecules

Inhibition of CDK Activity

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome ...

Chromosome Duplication

Cohesins

Condensins

The Mitotic Spindle

Centrosome Duplication

Spindle Assembly

Attachment of Sister Chromatids

Forces Acting on Chromosomes

Separation of Sister Chromatids

The Spindle Assembly Checkpoint

Anaphase A and B

Anaphase Promoting Complex

The Contractile Ring

The Phragmoplast

Distribution of Cytoplasmic Content

An Introduction to Cell Division

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Cell Cycle Analysis

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Live Cell Imaging of Mitosis

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Fertilization

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Cleavage and Blastulation

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Gastrulation

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Neurulation

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Cell Migration

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An Introduction to Developmental Genetics

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An Introduction to Organogenesis

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Fate Mapping

Fate mapping is a technique used to understand how embryonic cells divide, differentiate, and migrate during development. In classic fate mapping ...

Students who demonstrate understanding can use a model to illustrate the role of cellular division (mitosis) and differentiation in producing and maintaining complex organisms.  Assessment Boundary: Assessment does not include specific gene control mechanisms or rote memorization of the steps of mitosis.

DNA Packaging

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The ...

Organization of Genes

Structure of a Gene

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Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA ...

Chromosome Replication

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The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside ...

The Nucleosome Core Particle

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Nucleosome Remodeling

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Chromatin Packaging

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Karyotyping

Proteins: From Genes to Degradation

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA ...

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between ...

Mutations

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genome-size-and-the-evolution-of-new-genes

Genome Size and the Evolution of New Genes

Gene Families

Students who demonstrate understanding can ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring. Assessment Boundary: Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

3.1.9-12.P

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the ...

Binary Fission

Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the ...

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the ...

What is Meiosis?

Meiosis is the process by which diploid cells divide to produce haploid daughter cells. In humans, each diploid cell contains 46 chromosomes, half from ...

Meiosis I

Meiosis is a carefully orchestrated set of cell divisions, the goal of which&#x2014;in humans&#x2014;is to produce haploid sperm or eggs, each containing ...

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 ...

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where ...

Students who demonstrate understanding can make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Assessment Boundary: Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

3.1.9-12.Q

Spindle Position and Cell Division Plane

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