Name: cytoplasmic content during cell division
Uploaded: 2023-04-30T18:28:51.000Z
Duration: 2 min 33 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Distribution of Cytoplasmic Content

Das Zytoplasma einer Zelle enthält viele verschiedene Organellen. Jede Organelle unterscheidet sich in ihrer Struktur, Funktion und Kopienzahl; Daher muss eine Zelle unterschiedliche Strategien anwenden, um jeden Organellentyp richtig in seine Tochterzellen zu unterteilen.
Die Kernhülle und das endoplasmatische Retikulum sind direkt miteinander verbunden, und daher ist ihre Verteilung an die Tochterzellen gekoppelt.
In der Prophase verschmelzen die Kernhülle und die zugehörigen Proteine mit dem endoplasmatischen Retikulum, wobei sich das blattförmige endoplasmatische Retikulum in eine röhrenförmige Struktur verwandelt und sich während der Metaphase in der Nähe der Zellrinde neu organisiert.
Nach der Chromosomensegregation umgibt das endoplasmatische Retikulum die Schwesterchromatiden und reformiert die Kernhülle.
Bei der Mitose werden die Mitochondrien gespalten, um fragmentierte Mitochondrien zu produzieren, die sich in der gesamten Zelle ausbreiten.
Es wird angenommen, dass diese fragmentierten Mitochondrien während der Zellteilung durch einen stochastischen oder zufälligen Prozess an die Tochterzellen verteilt werden und dann fusionieren, um ihre Form und Größe wiederzuerlangen.
Eine einzige Kopie des Golgi-Körpers ist in der Zelle vorhanden, und ihre gleichmäßige Verteilung ist für das Überleben der Zellen von entscheidender Bedeutung. Der Golgi-Körper zerlegt sich schrittweise, bevor er sich in die Tochterzellen aufteilt.
Das Golgi-Band mit den gestapelten Zisternen zerfällt in mehrere Mini-Stapel, die dann weiter in Vesikel zerlegt werden.
In der Anaphase verschmelzen die Golgi-Vesikel zu Zisternen, die dann wieder gestapelt werden. Dann bilden sich in der Telophase kleine und große Zwillings-Golgis in der Nähe des Mittelkörpers bzw. des Zentrosoms.
Während der Zytokinese verschmelzen schließlich die kleineren Zwillings-Golgi-Körper mit den größeren und bilden in jeder Tochterzelle den einzelnen Golgi-Körper.
Eine symmetrische Teilung findet in den meisten Zellen statt, bei denen eine Zelle zwei identische Tochterzellen mit dem gleichen Schicksal hervorbringt.
In einigen Zellen kommt es zu einer asymmetrischen Teilung, bei der die Determinanten des Zellschicksals ungleich vererbt werden, was zur Produktion nicht identischer Tochterzellen führt.
Zum Beispiel können Stammzellen eine asymmetrische Zellteilung durchlaufen, um eine Tochterzelle zu produzieren, die sich wie die Elternstammzelle verhält, während die andere Tochterzelle einer anderen Zelllinie folgen kann.

<ol>
	<li>Rabouille, Catherine und Eija Jokitalo. "Teilung des Golgi-Apparats während der Zellteilung." Molekulare Membranbiologie 20, Nr. 2 (2003): 117-127.</li>
	<li>Yamano, Koji und Richard J. Youle. "Kopplung von Mitochondrien und Zellteilung." Natur Zellbiologie 13, Nr. 9 (2011): 1026.</li>
	<li>McCullough, Simon und John Lucocq. "Positionierung und Partitionierung des endoplasmatischen Retikulums in mitotischen HeLa-Zellen." Zeitschrift für Anatomie 206, Nr. 5 (2005): 415-425.</li>
</ol>

verteilung des zytoplasmatischen inhalts

Zytokinese trennt die Chromosomen und Organellen einer Zelle in ihre Tochterzellen. Organellen teilen sich und wachsen vor der Zellteilung, können aber nicht de novo synthetisiert werden; Daher müssen Zellen mindestens eine Kopie jeder Organelle erhalten, um zu überleben. Derzeit sind viele Einzelheiten der Verteilung der Organellen noch nicht vollständig geklärt.

Verteilung zytoplasmatischer Determinanten

Das Zytoplasma enthält verschiedene Organellen sowie Salze, Proteine und Wasser. Die Verteilung kleiner Organellen wie Peroxisomen und Lysosomen kann durch einfache Diffusion erklärt werden. Da jede Zelle über mehrere Kopien jeder Organelle verfügt, besteht eine hohe Wahrscheinlichkeit, dass Tochterzellen mindestens eine Kopie der Organelle erhalten. Allerdings erfordert die Verteilung großer Organellen wie des ER oder des Chloroplasten komplexere, evolutionär konservierte Mechanismen.

Jüngste Fortschritte bei der Verbreitung von Golgi-Körpern

Der Golgi-Apparat bricht zusammen, wenn die Zellen in die Mitose eintreten. Zwei mögliche Modelle könnten die Verteilung des Golgi-Apparats in Tochterzellen erklären. Nach dem ersten Modell werden während der Prophase die Golgi-Tubuli gespalten und kleine Golgi-Stapel freigesetzt. Diese sind weiter in Vesikel und Tubuli fragmentiert. Diese mitotischen Golgi-Cluster (MGC) verteilen sich im Zytoplasma und segregieren während der Anaphase in die sich teilenden Zellen. Spindelmikrotubuli steuern die Verteilung von MGC auf geordnete Weise. Das zweite Modell geht davon aus, dass die Golgi-Proteine und -Membranen zu Beginn der Mitose im ER resorbiert werden, was es dem Golgi ermöglicht, sich mit dem ER zu teilen.

Jüngste Fortschritte bei der Verteilung von Mitochondrien

Mitochondrien sind entscheidende Organellen für das Überleben und Wachstum der Zellen. Während der Mitose werden Mitochondrien in kleinere Fragmente geteilt, die sich dann zwischen den Tochterzellen aufteilen können. Ein konserviertes Protein DRP1 verengt die äußere Mitochondrienmembran und führt zu einer Mitochondrienspaltung. Cyclin B-Cdk1 und Aurora-A, zwei mitotische Kinasen, regulieren die Aktivierung von DRP1.

Jüngste Fortschritte bei der Verteilung des endoplasmatischen Retikulums

Das Interphase-Endoplasmatische Retikulum (ER) ist eine kontinuierliche Membran, die die Membranen für Golgi-Körper, Endolysosomen, Vesikel und die Plasmamembran synthetisiert. Während der Mitose kann es zu einer Fragmentierung des ER kommen oder es kann als Zisterne verbleiben. In HeLa-Zellen wird das ER in Form von Zisternen aufrechterhalten und ist auf die Zellrinde beschränkt. Das ER ist ungleichmäßig verteilt und die zentralen Zisternen sind mit der Spindel verbunden. Das ER kann an die Wände der mitotischen Zellrinde gebunden werden, bevor es an die Tochterzellen verteilt wird.

Verteilung des zytoplasmatischen Inhalts

Die Zytokinese trennt die Chromosomen und Organellen einer Zelle in ihre Tochterzellen. Organellen teilen und wachsen vor der Zellteilung, können aber nicht ...

Education

Lehre

JoVE Core

Cell Biology

Zellbiologie

Unit 1

Einheit 1

Cell Division

Zellteilung

KEY TERMS AND CONCEPTS

KERNBEGRIFFE UND KONZEPTE

cytoplasmic content during cell division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function.

The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split.

The video focuses on how this distribution occurs as part of cell division. It highlights the movement and sharing of cell material during the process. Understanding this step helps students see how one cell becomes two working cells.

Cytoplasmic Content During Cell Division

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

centrosome duplication

Centrosome Duplication in Cell Division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Chromatid Pulling in Cell Division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

Spindle Position and Cell Division Plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how cytoplasmic content is distributed during cell division and how daughter cells receive the materials needed to keep functioning.

Cytoplasmic Content During Cell Division | JoVE K12

cell biology

JoVE Core Zellbiologie veranschaulicht komplexe zelluläre Prozesse in leicht verständlichen und dennoch umfassenden animierten Videolektionen. Diese Sammlung konzentriert sich auf die wesentlichen Bestandteile und Funktionen von Zellen, einschließlich Organellen und Membranen, Zelltransport und Zellenergie. Ergänzt wird sie durch Videos von Wissenschaftlern in Aktion, die die Anwendung der Zellbiologie in realen Forschungsexperimenten demonstrieren.

Louisiana Student Standards for Science

High School

Life Sciences

from-molecules-to-organisms-structures-and-processes

From Molecules To Organisms: Structures And Processes

HS-LS1-4

Tennessee Academic Standards

Biology

From Molecules to Organisms: Structures and Processes

BIO1.LS1.6

Minnesota Academic Standards in Science

3.1.1 9L.3.1.1.2

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

Arizona Science Standards

HS+B.L1

HS+B.L1U1.9

Alabama Course of Study Science

9-12.B.1.3

Florida Department of Education

SC.912.L.16

SC.912.L.16.14

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

B-LS1-4.

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Interphase

The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the ...

The Cell Cycle Control System

Positive Regulator Molecules

Inhibition of CDK Activity

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome ...

Chromosome Duplication

Cohesins

Condensins

The Mitotic Spindle

Centrosome Duplication

Spindle Assembly

Attachment of Sister Chromatids

Forces Acting on Chromosomes

Separation of Sister Chromatids

The Spindle Assembly Checkpoint

Anaphase A and B

Anaphase Promoting Complex

The Contractile Ring

Determining the Plane of Cell Division

The Phragmoplast

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Cell Migration

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Fate Mapping

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Students who demonstrate understanding can use a model to illustrate the role of cellular division (mitosis) and differentiation in producing and maintaining complex organisms.  Assessment Boundary: Assessment does not include specific gene control mechanisms or rote memorization of the steps of mitosis.

DNA Packaging

DNA as a Genetic Template

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Organization of Genes

Structure of a Gene

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Chromosome Structure

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The centromere is a DNA ...

Chromosome Replication

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The Nucleosome

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Karyotyping

Proteins: From Genes to Degradation

The DNA Helix

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The Central Dogma

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RNA is the Missing Link Between ...

Mutations

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genome-size-and-the-evolution-of-new-genes

Genome Size and the Evolution of New Genes

Gene Families

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3.1.9-12.P

Genomic DNA in Prokaryotes

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Binary Fission

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Genomic DNA in Eukaryotes

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What is Meiosis?

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Meiosis I

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Meiosis II

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Crossing Over

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Students who demonstrate understanding can make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Assessment Boundary: Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

3.1.9-12.Q

Cytoplasmic Content During Cell Division

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