Name: raman spectra and molecular fingerprints
Uploaded: 2024-12-05T18:32:10.000Z
Duration: 1 min 20 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Physical Science

Waves and Electromagnetic Radiation

3.2.9-12.X

Raman Spectroscopy: Overview

Der Raman-Effekt tritt in Molekülen auf, deren Polarisierbarkeit der Nettobindung sich während der Schwingung ändert. Peaks innerhalb des Spektrums stellen spezifische Schwingungsmoden von Molekülen dar und liefern einen einzigartigen Fingerabdruck für das Verständnis der molekularen Zusammensetzung und Bindung.
Bei der Raman-Spektroskopie interagiert ein hochenergetischer monochromatischer Laser mit einer Probe. Der größte Teil des Lichts wird durch ihn hindurch durchgelassen, aber ein kleiner Teil streut in verschiedene Richtungen, was als Funktion der Wellenlänge oder Wellenzahl aufgezeichnet wird.
Die Frequenz des gestreuten Photons kann entweder mit der Frequenz des einfallenden Photons übereinstimmen, was als Rayleigh-Streuung bezeichnet wird, oder eine Frequenzverschiebung aufweisen, die als Raman-Streuung bekannt ist.
Die Raman-Streuung besteht aus der Stokes-Streuung und der Anti-Stokes-Streuung, wobei gestreute Photonen niedrigere Frequenzen bzw. höhere Frequenzen als die einfallenden Photonen haben. 
Die Intensität der Raman-Peaks hängt von der Polarisierbarkeit der Bindung, der Laserintensität und der Konzentration der Raman-aktiven Gruppen ab.
Stokes-Signale sind jedoch fast immer stärker als Anti-Stokes-Signale, da sich die Moleküle unter den meisten Bedingungen im schwingungsbedingten Grundzustand befinden.

raman-spektroskopie: Übersicht

Das Grundprinzip der Raman-Spektroskopie basiert auf der Wechselwirkung zwischen Licht und Materie, insbesondere auf der unelastischen Streuung von Photonen durch Moleküle. Wenn ein monochromatischer Lichtstrahl, typischerweise von einer Laserquelle, mit einer Probe interagiert, hat der Großteil des gestreuten Lichts dieselbe Frequenz wie das einfallende Licht. Dies wird als Rayleigh-Streuung bezeichnet.

Ein kleiner Teil des gestreuten Lichts weist jedoch eine Frequenzverschiebung aufgrund des Energieaustauschs zwischen den einfallenden Photonen und den Schwingungsenergieniveaus der Moleküle in der Probe auf. Dieses Phänomen wird als Raman-Streuung bezeichnet.

Um Raman-Spektren zu erhalten, wird die Probe mit einem monochromatischen Laser bestrahlt. Die gestreute Strahlung wird in einem geeigneten Winkel (normalerweise 180°) mithilfe eines Spektrometers mit einem empfindlichen Detektor gemessen. Die Intensität des frequenzverschobenen gestreuten Lichts als Funktion der Wellenlänge oder Wellenzahl liefert das Raman-Spektrum der Probe.

Resonanz-Raman-Streuung tritt auf, wenn die Anregungswellenlänge nahe an einem elektronischen Übergang des Moleküls liegt. Dies erhöht die Raman-Streuintensität und erleichtert die Erkennung selbst schwacher Signale. Die Wahl einer geeigneten Anregungswellenlänge ist jedoch entscheidend, um Fluoreszenzstörungen zu vermeiden, die das Raman-Signal überlagern können. In vielen Fällen werden Nahinfrarot- oder sichtbare Laser verwendet, um die Fluoreszenz zu minimieren.

Wenn ein Molekül ein Photon absorbiert, wird es vorübergehend auf ein virtuelles Energieniveau angehoben. Dieser virtuelle Zustand ist nur von kurzer Dauer und das Molekül kehrt durch Emission eines Photons schnell auf ein niedrigeres Energieniveau zurück. Der Energieunterschied zwischen den einfallenden und den emittierten Photonen entspricht der Energiedifferenz der Schwingungsniveaus des Moleküls.

Es gibt zwei Arten der Raman-Streuung: Stokes- und Anti-Stokes-Streuung. Bei der Stokes-Streuung hat das emittierte Photon eine geringere Energie (längere Wellenlänge) als das einfallende Photon, während bei der Anti-Stokes-Streuung das emittierte Photon eine höhere Energie (kürzere Wellenlänge) hat.

Die Frequenzverschiebungen der unelastisch gestreuten Strahlung entsprechen der Schwingungsfrequenz des Moleküls. Durch Analyse dieser Frequenzverschiebungen können wertvolle Informationen über die Molekülstruktur und chemische Zusammensetzung der Probe gewonnen werden.

Raman-Spektren werden typischerweise als Diagramme der Intensität gegenüber der Wellenzahl (Kehrwert der Wellenlänge) dargestellt. Die Peaks im Spektrum repräsentieren spezifische Schwingungsformen der Moleküle in der Probe und liefern einen einzigartigen „Fingerabdruck“ zur Identifizierung.

Betrachtet wird eine Probe von Tetrachlorkohlenstoff. Bei Bestrahlung mit einem monochromatischen Laser treten sowohl Stokes- als auch Anti-Stokes-Streuung auf. Die Intensität des Stokes-Signals ist stärker als die des Anti-Stokes-Signals, da die Population der Moleküle im Grundzustand der Schwingung höher ist als die im angeregten Zustand bei Raumtemperatur.

Die Schwingungsformen können durch Analyse des Raman-Spektrums von Tetrachlorkohlenstoff identifiziert werden. Die mit diesen Schwingungsformen verbundenen Frequenzverschiebungen liefern wertvolle Informationen über die chemische Zusammensetzung und Bindung des Moleküls.

Raman-Spektroskopie: Übersicht

Das zugrundeliegende Prinzip der Raman-Spektroskopie beruht auf der Wechselwirkung zwischen Licht und Materie, genauer gesagt auf der inelastischen Streuung von ...

Education

Lehre

JoVE Core

Analytical Chemistry

Analytische Chemie

Unit 1

Einheit 1

Molecular Vibrational Spectroscopy

Molekülschwingungsspektroskopie

KEY TERMS AND CONCEPTS

ZENTRALE BEGRIFFE UND KONZEPTE

raman spectra and molecular fingerprints

Raman spectroscopy uses laser light to study how molecules scatter light and reveal their vibrational energy levels. A monochromatic beam, or single-color light, hits the sample first. Most of the scattered light keeps the same frequency as the incoming light. This is called Rayleigh scattering. A small part of the light shifts in frequency because the photons exchange energy with molecular vibrations. That shifted light is Raman scattering.

To collect a Raman spectrum, the sample is illuminated with laser light and the scattered radiation is gathered at a suitable angle, often 180 degrees. A spectrometer with a sensitive detector measures the shifted light. The result is a Raman spectrum, which shows intensity against wavelength or wavenumber. The peaks in this plot come from specific vibrational modes in the sample.

Some Raman signals are stronger when the laser wavelength is close to an electronic transition. This is called resonance Raman scattering. It can make weak signals easier to detect. The choice of excitation wavelength also matters because fluorescence can interfere with the Raman signal. In many cases, visible or near-infrared lasers are used to reduce that problem.

Raman scattering involves a very short-lived virtual energy level. The molecule briefly moves through this state and then returns to a vibrational level by emitting a photon. The energy difference between the incoming and outgoing photons matches the molecule’s vibrational energy. Two common forms are Stokes scattering and anti-Stokes scattering. In Stokes scattering, the emitted photon has lower energy and a longer wavelength. In anti-Stokes scattering, the emitted photon has higher energy and a shorter wavelength.

The frequency shifts in a Raman spectrum give useful clues about molecular structure and chemical composition. Because each sample has its own set of vibrational peaks, the spectrum can act like a unique fingerprint for identification. Carbon tetrachloride is one example. When it is irradiated with monochromatic laser light, both Stokes and anti-Stokes scattering occur. The Stokes signal is stronger than the anti-Stokes signal because more molecules are in the ground vibrational state at room temperature. By analyzing these peaks, the vibrational modes of carbon tetrachloride can be identified, along with information about its bonding and composition.

Raman Spectra and Molecular Fingerprints

infrared (ir) spectroscopy: overview

IR Spectra and Functional Group ID

Infrared (IR) spectroscopy helps identify functional groups in molecules by measuring how they absorb infrared light. It is based on the idea that electromagnetic radiation can move atoms or molecules from a lower energy state to a higher one when the radiation matches the energy gap between those states. In IR spectroscopy, that absorption happens as molecular vibrations change.

ir spectroscopy: molecular vibration overview

ir-spectroscopy-molecular-vibration-overview

IR Spectroscopy: Vibration Types and Bands

IR spectroscopy explains how covalently bonded molecules absorb infrared radiation by moving to higher vibrational levels. The key motions are stretching and bending vibrations. These motions help describe how a bond responds when it absorbs IR energy. Stretching vibrations happen along the bond line and change the distance between two bonded atoms. They can be symmetric, where two bonds lengthen and shorten together, or asymmetric, where one bond moves one way while another moves the opposite...

ir spectroscopy: hooke's law approximation of molecular vibration

ir-spectroscopy-hooke-s-law-approximation-of-molecular-vibration

Molecular Bond Vibrations in IR Spectroscopy

Molecular bond vibrations can be modeled with IR spectroscopy using Hooke’s law. A covalently bonded heteronuclear diatomic molecule is treated like two masses connected by a spring. The atoms act like the masses, and the bond acts like the spring. Hooke’s law links the vibrational frequency to two main factors. The frequency increases with the force constant, K, which describes bond stiffness. It decreases with the reduced mass, μ, which combines the masses of both atoms into one value for...

ir spectrometers

IR Spectrometer Signal Paths

IR spectrometers use infrared light to compare a sample beam with a reference beam and record how each one behaves. The two main instruments are dispersive IR spectrometers and Fourier transform infrared, or FTIR, spectrometers. In a dispersive IR spectrometer, a hot wire produces the infrared beam. Mirrors split it into two equal beams. One beam passes through the sample, and the other serves as the reference. The beams then move into the monochromator, which separates the radiation into a...

ir spectrum

IR Spectrum Peak Regions and Patterns

Infrared (IR) spectroscopy shows how a molecule responds when IR radiation passes through it. The bonds in the molecule stretch or bend as they absorb that energy. This creates an absorption spectrum, which plots percent transmittance against wavenumber. Transmittance is the ratio of radiant power that passes through a sample to the radiant power from the source. When transmittance is multiplied by 100, it becomes percent transmittance, or %T. %T ranges from 100% for no absorption to 0% for...

ir absorption frequency: hybridization

C-H Stretching in Alkane, Alkene, and Alkyne IR

Hydrocarbons such as alkanes, alkenes, and alkynes show characteristic C–H stretching bands in infrared, or IR, spectra. These bands help identify how the carbon atoms are hybridized. The bond type affects the stretching frequency that appears in the spectrum. The difference comes from s character, which is the amount of s-orbital contribution in a hybrid orbital. Among sp, sp2, and sp3 hybridized orbitals, sp has the most s character at 50%. Because of that, the electrons are held closer to...

ir absorption frequency: delocalization

Conjugation Shifts IR Bond Stretching

Conjugation shifts IR bond stretching because electron delocalization changes bond strength. Electron delocalization means electrons spread across more than one atom instead of staying in one bond or one atom. It is common in conjugated systems, where alternating single and double bonds let pi-electrons move through the molecule. This electron movement helps stabilize the molecule. It also lowers the double bond character of the bonds in the conjugated network. When a bond has less double bond...

ir frequency region: x&ndash;h stretching

IR Peaks for C-H, O-H, and N-H Bonds

IR spectroscopy shows the stretching bands of C-H, O-H, and N-H bonds in the 2700-4000 cm-1 range. These X-H bonds are useful for identifying common functional groups in a spectrum. Their peaks appear in different parts of this region and often have different shapes and strengths. C-H stretching gives sharp bands in the 2850-3000 cm-1 range. O-H stretching appears in the 3650-3200 cm-1 range, and N-H stretching appears in the 3500-3100 cm-1 range. N-H stretching usually shows one or two sharp...

ir frequency region: alkyne and nitrile stretching

ir-frequency-region-alkyne-and-nitrile-stretching

Triple-Bond Signals in IR Spectra

Alkyne and nitrile groups both contain triple bonds, and they show clear stretching bands in the diagnostic IR region. These absorptions usually appear around 2100 to 2300 cm −1 . This makes them useful markers when reading an IR spectrum. Triple bonds absorb at a higher stretching frequency than double bonds and single bonds. The C≡C bond stretches at a higher frequency than a C=C or C–C bond. The C≡N bond also stretches at a higher absorption than C=N and C–N bonds. This higher stretching...

ir frequency region: alkene and carbonyl stretching

ir-frequency-region-alkene-and-carbonyl-stretching

IR Signals for C=C and C=O Bonds

IR spectroscopy helps identify alkene double bonds and carbonyl groups by their stretching signals. These bonds absorb in a diagnostic region of the IR spectrum. Alkenes also show vinylic C–H stretching and C–H out-of-plane bending bands. Those bands can help determine substitution patterns on the double bond. The exact stretching frequency can change with bond environment. Resonance, inductive effects, ring strain, dipole moment, and hydrogen bonding can all shift the absorption. That is why...

ir frequency region: fingerprint region

IR Spectra: Diagnostic vs Fingerprint Region

IR spectra are divided into a diagnostic region and a fingerprint region. The diagnostic region lies above 1500 cm −1 and appears on the left side of the spectrum. It shows key bond stretches that help identify functional groups. In this region, single-bond vibrations from N-H, C-H, and O-H stretches appear at higher wavenumbers. The C≡C and C≡N stretches occur between 2100 and 2300 cm −1. The C=O, C=N, and C=C stretches appear between 1600 and 1850 cm −1. The fingerprint region lies below...

ir spectrum peak intensity: amount of ir-active bonds

ir-spectrum-peak-intensity-amount-of-ir-active-bonds

IR Peak Intensity and Bond Dipole Change

IR peak intensity depends on how much a bond’s dipole moment changes during vibration. When infrared light passes through a molecule, absorption happens only when a stretching or bending motion causes a substantial change in that bond dipole moment. That is what makes a molecule infrared active. Vibrational transitions usually fall in the infrared region from 4000 to 400 cm-1. Molecular bonds can stretch and bend in different ways, so an IR spectrum shows peaks with different intensities. The...

ir spectrum peak intensity: dipole moment

IR Absorption and Bond Dipole Changes

IR absorption depends on whether a bond has a changing dipole moment. A dipole moment comes from partial charge on each atom and the distance between them. It helps determine how strongly a bond absorbs infrared light and how intense the peak looks. A bond in an electric field can be stretched or compressed depending on the field direction. Infrared radiation has an electric field that flips direction very quickly. So polar bonds are pulled back and forth as the field reverses. If this motion...

ir spectrum peak broadening: hydrogen bonding

ir-spectrum-peak-broadening-hydrogen-bonding

Hydrogen Bonding Effects in IR O-H Peaks

Hydrogen bonding changes the O-H stretching peak in IR spectroscopy. The bond’s vibrational frequency depends on bond strength. Stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. In very dilute alcohol or phenol samples, or in the gas phase, the strong O-H bond gives a sharp peak at 3600-3650 cm−1. When intermolecular hydrogen bonding or intramolecular hydrogen bonding weakens the O-H bond, the stretch shifts to about 3300-3400 cm−1. This range of...

ir spectrum peak splitting: symmetric vs asymmetric vibrations

ir-spectrum-peak-splitting-symmetric-vs-asymmetric-vibrations

IR Peak Splitting in Amines and Nitro Groups

IR peak splitting can reveal how identical bonds in a polyatomic group are moving. These bonds may stretch in phase, which is symmetric stretching, or out of phase, which is asymmetric stretching. The two motions often create different IR peak shapes and positions. As a general rule, asymmetric stretching appears at a higher frequency than symmetric stretching. This pattern is useful for spotting functional groups in an IR spectrum. It also helps explain why some groups show one peak while...

applications of ir spectroscopy: overview

IR Spectroscopy Uses in Science and Forensics

IR spectroscopy is a useful tool for finding out what materials are made of. It is non-destructive, so the sample can often be kept intact. It gives chemical information about functional groups and the bonds between atoms in a molecule. This makes IR spectroscopy helpful in organic synthesis, pharmaceutical research, and materials science. Scientists use it to identify and characterize organic and inorganic compounds. It also helps with structural elucidation, which means working out a...

attenuated total reflectance (atr) infrared spectroscopy: overview

attenuated-total-reflectance-atr-infrared-spectroscopy-overview

ATR Infrared Spectroscopy for Material ID

ATR infrared spectroscopy is a method for identifying the composition of materials. It is used in chemistry, materials science, forensic science, and other areas where sample characterization is needed. The technique is useful because it usually needs little to no sample preparation and can be applied to many kinds of samples. The process starts when infrared, or IR, radiation is directed onto a diamond or germanium crystal. Because this crystal has a high refractive index, the IR beam...

raman spectroscopy: overview

Raman spectroscopy uses laser light to study how molecules scatter light and reveal their vibrational energy levels. A monochromatic beam, or single-color light, hits the sample first. Most of the scattered light keeps the same frequency as the incoming light. This is called Rayleigh scattering. A small part of the light shifts in frequency because the photons exchange energy with molecular vibrations. That shifted light is Raman scattering. To collect a Raman spectrum, the sample is...

raman spectroscopy instrumentation: overview

raman-spectroscopy-instrumentation-overview

Raman Spectroscopy Setup and Detectors

Raman spectroscopy uses a laser, a sample holder, a wavelength selector, and a detector to measure scattered light from a sample. The laser is monochromatic, meaning it has one main wavelength. It usually uses visible or near-infrared light to create a tightly focused beam. That beam hits the sample and some of the light scatters. Liquid and gas samples are often placed in ordinary glass capillaries. Solid samples can be tested as powders packed into capillaries or as potassium bromide pellets.

key terms and concepts

Learn how Raman spectroscopy uses laser light, Stokes and anti-Stokes scattering, and spectra peaks to identify molecules like carbon tetrachloride.

Raman Spectra and Molecular Fingerprints | JoVE K12

analytical chemistry

JoVE Core Analytical Chemistry simplifies complex chemical principles by breaking them down into clear, engaging animations, making topics from SI Units to NMR spectroscopy more accessible and straightforward. Additionally, the scientist-in-action videos highlight the application of these concepts in real-world research conducted in laboratories globally.

Travelling Waves

A wave is a disturbance that propagates from its source, repeating itself periodically, and is typically associated with simple harmonic motion. ...

Wave Parameters

The simplest mechanical waves are associated with simple harmonic motion and repeat themselves for several cycles. These simple harmonic waves can be ...

Equations of Wave Motion

Mathematically, the motion of a wave can be studied using a wavefunction. Consider a string oscillating up and down in simple harmonic motion, having a ...

Graphing the Wave Function

Consider the wave equation for a sinusoidal wave moving in the positive x-direction. The wave equation is a function of both position and time. From the ...

/files/media/science-education/science-education-thumbs/14721.jpg

Velocity and Acceleration of a Wave

A wave propagates through a medium with a constant speed, known as a wave velocity. It is different from the speed of the particles of the medium, which ...

Speed of a Transverse Wave

The speed of a wave depends on the characteristics of the medium. For example, in the case of a guitar, the strings vibrate to produce the sound. The ...

/files/media/science-education/science-education-thumbs/14722.jpg

problem-solving-tuning-of-a-guitar-string

Problem-Solving: Tuning of a Guitar String

In the case of stringed instruments like the guitar, the elastic property that determines the speed of the sound produced is its linear mass density or ...

/files/media/science-education/science-education-thumbs/14744.jpg

Kinetic and Potential Energy of a Wave

All forms of waves carry energy; this is directly visualized in nature. For instance, the waves of earthquakes are so intense that they can shake huge ...

Energy and Power of a Wave

The total energy associated with a wavelength is the sum of the potential energy and the kinetic energy. The average rate of energy transfer associated ...

Students who demonstrate understanding can use mathematical representations to support a claim regarding relationships among the frequency, wavelength, and speed of waves traveling in various media. Clarifying Statement: Examples of data could include electromagnetic radiation traveling in a vacuum and glass, sound waves traveling through air and water, and seismic waves traveling through the Earth. Assessment Boundry: Assessment is limited to algebraic relationships and describing those relationships qualitatively.

3.2.9-12.T

Interference and Superposition of Waves

When two waves of the same nature occur in the same region simultaneously, they result in interference. Interference of waves implies that the net effect ...

Sound Waves: Interference

Sound waves can be modeled either as longitudinal waves, wherein the molecules of the medium oscillate around an equilibrium position, or as pressure ...

Interference: Path Lengths

Consider two sources of sound, that may or may not be in phase, emitting waves at a single frequency, and consider the frequencies to be the same.
Two ...

/files/media/science-education/science-education-thumbs/14727.jpg

Sound Waves: Resonance

Resonance is produced depending on the boundary conditions imposed on a wave. Resonance can be produced in a string under tension with symmetrical ...

Electromagnetic Waves

James Clerk Maxwell formulated a single theory combining all the electric and magnetic effects scientists knew during that time, calling the phenomena his ...

The Electromagnetic Spectrum

Electromagnetic waves are categorized according to their wavelengths and frequencies, giving the electromagnetic spectrum. These waves are classified as ...

Electromagnetic Wave Equation

Maxwell&#39;s equations for electromagnetic fields are related to source charges, either static or moving. These fields act on a test charge, whose ...

propagation-speed-of-electromagnetic-waves

Propagation Speed of Electromagnetic Waves

Electromagnetic waves are consistent with Ampere&#39;s law. Assuming there is no conduction current Ampere&#39;s law is given as:


Consider a plane ...

Electromagnetic Waves in Matter

Electromagnetic waves can travel in the vacuum as well as in matter. For example light, which is an electromagnetic wave, can travel through air, water, ...

Energy Carried By Electromagnetic Waves

Anyone who has used a microwave oven knows there is energy in electromagnetic waves. Sometimes, this energy is obvious, such as in the summer sun&#39;s ...

Intensity Of Electromagnetic Waves

The energy transport per unit area per unit time, or the Poynting vector, gives the energy flux of an electromagnetic wave at any specific time. For a ...

Momentum And Radiation Pressure

An object absorbing an electromagnetic wave would experience a force in the direction of propagation of the wave. This force occurs because ...

Radiation Pressure: Problem Solving

The radiation pressure applied by an electromagnetic wave on a perfectly absorbing surface equals the energy density of the wave. The wave&#39;s momentum ...

Students who demonstrate understanding can evaluate the claims, evidence, and reasoning behind the idea that electromagnetic radiation can be described either by a wave model or a particle model and that for some situations one model is more useful than the other.  Clarifying Statement: Emphasis is on how the experimental evidence supports the claim and how a theory is generally modified in light of new evidence.  Examples of a phenomenon could include resonance, interference, diffraction, and photoelectric effect.  Assessment Boundry: Assessment does not include using quantum theory.

3.2.9-12.V

Absorption of Radiation

The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:

where &#963; is the Stefan-Boltzmann constant, a ...

Students who demonstrate understanding can evaluate the validity and reliability of claims in published materials of the effects that different frequencies of electromagnetic radiation have when absorbed by matter.  Clarifying Statement: Emphasis is on the idea that photons associated with different frequencies of light have different energies, and the damage to living tissue from electromagnetic radiation depends on the energy of the radiation.  Examples of published materials could include trade books, magazines, web resources, videos, and other passages that may reflect bias.  Assessment Boundry: Assessment is limited to qualitative descriptions.

3.2.9-12.W

Energy Transfer in Chemical Reactions

Chemical reactions require sufficient energy to cause the matter to collide with enough precision and force that old chemical bonds can be broken and new ...

Dye-sensitized Solar Cells

Source: Tamara M. Powers, Department of Chemistry, Texas A&#38;M University
Today&#39;s modern world requires the use of a large amount of energy. While ...

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms ...

The electromagnetic spectrum consists of all the types of electromagnetic radiation arranged according to their frequency and wavelength. Each of the ...

Photoelectric Effect

When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of ...

dual-nature-of-electromagnetic-em-radiation

Dual Nature of Electromagnetic (EM) Radiation

Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually ...

interaction-of-em-radiation-with-matter-spectroscopy

Interaction of EM Radiation with Matter: Spectroscopy

Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in ...

molecular-spectroscopy-absorption-and-emission

Molecular Spectroscopy: Absorption and Emission

Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational ...

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a ...

ultraviolet-and-visible-uvvis-spectroscopy-overview

Ultraviolet and Visible (UV&ndash;Vis) Spectroscopy: Overview

Ultraviolet&#8211;visible (UV&#8211;visible or UV&#8211;Vis) spectroscopy is an analytical technique that investigates the interaction between matter and ...

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation ...

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules&#39; inelastic scattering of ...

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes ...

Nuclear Magnetic Resonance (NMR): Overview

Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain ...

Students who demonstrate understanding can communicate technical information about how some technological devices use the principles of wave behavior and wave interactions with matter to transmit and capture information and energy.  Clarifying Statement: Examples could include solar cells capturing light and converting it to electricity; medical imaging; and communications technology.  Assessment Boundry: Assessments are limited to qualitative information.  Assessments do not include band theory.

Raman Spectra and Molecular Fingerprints

Description

Transcript

Tags

More videos by Standards