Protocol
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
Syrena C. Fernandes, Daniel J. Wilson, Charles R. Mace
Department of Chemistry, Tufts University
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
Josef Pannee1,2, Kaj Blennow1,2, Henrik Zetterberg1,2,3, Erik Portelius1,2
1Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3UCL Institute of Neurology, Queen Square
A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Laura B. Goodman1, Renee R. Anderson1, Marcia Slater2, Elen Ortenberg2, Randall W. Renshaw1, Brittany D. Chilson1, Melissa A. Laverack1, John S. Beeby1, Edward J. Dubovi1, Amy L. Glaser1
1Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.
High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.
Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation
Ke Ma, Dominique P. Lippelt, Bernhard Hommel
Cognitive Psychology Unit, Leiden University
Here, we describe virtual-hand and virtual-face illusion paradigms that can be used to study body-related self-perception/-representation. They have already been used in various studies to demonstrate that, under specific conditions, a virtual hand or face can be incorporated into one's body representation, suggesting that body representations are rather flexible.
Disclosures
No conflicts of interest declared.