Afm Wet Cell
Afm Wet Cell - Related Videos
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
08:21
Quantitative Hardness Measurement by Instrumented AFM-indentation
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Cited by 6 •
2016
This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.
06:15
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
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Cited by 9 •
2016
Cell-adhesiveness is key to many approaches in biomaterial research and tissue engineering. A step-by-step technique is presented using wet-chemistry for the surface modification of the important polymer PTFE with peptides.
08:29
Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
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Cited by 12 •
2016
To obtain basic information on the sorption and recycling of gold from aqueous systems the interaction of Au(III) and Au(0) nanoparticles on S-layer proteins were investigated. The sorption of protein polymers was investigated by ICP-MS and that of proteinaceous monolayers by QCM-D. Subsequent AFM enables the imaging of the nanostructures.
10:26
AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
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Cited by 39 •
2014
We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.
FAQs
Within the liquid chamber, a sharp probe scans across the sample while attached to a flexible cantilever. A laser detects cantilever deflection as the probe encounters the surface, and feedback adjusts the tip–sample force during scanning. This coordinated measurement produces nanoscale information without requiring the hydrated specimen to be dried first.
Force control helps regulate how strongly the probe interacts with the sample during scanning. Maintaining the selected interaction allows the instrument to collect surface information while also detecting responses related to mechanical behavior. In bioengineering studies, this supports mechanical mapping of cell membranes, biomolecules, biomaterials, and engineered surfaces under liquid conditions.
Hydration helps preserve biological and other water-dependent samples in conditions closer to their native state. A controlled solution environment also provides a way to maintain the surrounding conditions during measurement. Together, these features reduce drying-related changes and make the resulting structural and mechanical observations more relevant to hydrated biological interfaces.
The sample is placed in a liquid-compatible chamber, the chamber maintains the surrounding solution, and a probe mounted on a flexible cantilever is positioned above the surface. A laser monitors cantilever deflection as scanning proceeds, while feedback regulates tip–sample force. The instrument then records nanoscale topographic and mechanical information from the scan.
Wet-cell AFM can generate both nanoscale topographic maps and mechanical maps. Topographic data describe surface structure, whereas mechanical measurements reveal how regions respond to probe interaction. Examining these outputs together can distinguish structural features from differences in mechanical behavior, supporting analysis of hydrated samples and bioengineered surfaces.
The approach is useful when researchers need structural or mechanical information from hydrated biological systems or engineered interfaces. Applications identified for the method include studying cell membranes, biomolecules, biomaterials, tissue interfaces, biosensors, and bioengineered surfaces. Its compatibility with near-native conditions also supports investigations involving live samples and cellular engineering.