Ex Vivo Technique
Ex Vivo Technique - Related Videos
04:13
A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies
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2025
This video demonstrates a technique to study the in vivo localization of tumor-specific antibodies in a mouse tumor xenograft model. Upon subcutaneously injecting cancer cells into mice for tumor formation, fluorescently-labeled antibodies are injected that bind to tumor-specific receptors, enabling in vivo assessment of antibody localization via imaging.
04:38
An Ex Vivo Technique to Manufacture Chimeric Antigen Receptor T Cells
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2025
This video demonstrates a technique to manufacture chimeric antigen receptor (CAR) T cells ex vivo. Human T cells are incubated with magnetic beads coated with anti-CD3 and anti-CD28 antibodies, along with a medium supplemented with interleukin-2 (IL-2), leading to T cell activation and proliferation. Transgenic lentiviral vectors are introduced, allowing the transgenic RNA to integrate into the host genome and express the transgene. This results in the transduced T cells expressing chimeric...
05:30
Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model
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2025
This video shows an ex vivo technique for isolated lung perfusion. It is a tool for pulmonary research that will aid studies of various physiological and pathological mechanisms involved in lung transplantation procedures.
02:44
An In Vivo Technique to Track Bispecific Antibody-Induced T Cell Trafficking to Tumors
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2025
This video demonstrates a method for tracking bispecific antibody-induced T cell trafficking to tumors in a mouse model. Luciferase-transduced effector T cells are injected into a mouse with a subcutaneous tumor cell xenograft. Bispecific antibodies are then introduced, binding to T cells and the tumor cells. The localization of the T cells is tracked using bioluminescent imaging, with the magnitude of the signal indicating the extent of T cell infiltration into the tumor.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
FAQs
Maintaining biological viability is central to an ex vivo experiment. After isolation, cells, tissues, or organs must receive regulated temperature, nutrients, gases, and culture media appropriate to the sample. These conditions support ongoing function, allowing researchers to distinguish responses to a defined treatment from changes caused by loss of biological activity.
Ex vivo models preserve features of the original organism while adding experimental control. Unlike in vivo work, investigators can apply a defined treatment and directly measure tissue responses outside the body. Compared with in vitro experiments, the approach can retain cells within removed tissues or organs, helping connect controlled manipulation with biologically relevant responses.
The choice of sample determines what can be examined. Isolated cells, tissues, and organs offer different levels of biological organization, while culture media and regulated gases, nutrients, and temperature help sustain the selected material. Matching the controlled environment to the sample is therefore essential for meaningful measurements of disease mechanisms, drug effects, or therapeutic responses.
A basic workflow begins with isolating the target material from the organism, then placing it in a suitable laboratory environment. Researchers maintain viability with controlled temperature, nutrients, gases, and culture media before applying a defined treatment or recording measurements. This sequence enables direct assessment of how the sample responds under standardized experimental conditions.
Ex vivo technique is useful when researchers need controlled access to biological material without limiting the investigation to isolated cells. Applications described for the method include studying disease mechanisms, evaluating drug effects, investigating therapeutic strategies, supporting tissue engineering, and informing transplantation research. These uses make it relevant across biological techniques and translational research.
When samples retain features of the original organism, ex vivo models can support personalized medicine by enabling responses to be examined in material connected to an individual source. The same framework also assists transplantation research, where preserved biological function and controlled testing are relevant to evaluating therapeutic strategies.