25-gauge Transvitreal Pars Plana

25-gauge transvitreal pars plana is a minimally invasive ocular access technique that uses a small-gauge instrument to reach the vitreous and posterior eye. The instrument enters through the pars plana, a surgical entry region behind the iris, and passes through the vitreous to deliver material or perform targeted procedures while avoiding direct passage through the lens. In biology and ophthalmic research, this approach supports intravitreal delivery, retinal disease models, imaging, and vitreoretinal interventions. Precise control of the entry site, depth, sterility, and injection volume promotes reproducible results while helping limit tissue disruption and procedure-related complications.

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JoVE Core - Physics

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Most pressure gauges, like those on scuba tanks, are calibrated to read zero at atmospheric pressure. Readings from such gauges are called the gauge pressure, which is the pressure relative to atmospheric pressure. When the pressure inside the tank exceeds atmospheric pressure, the gauge reports a positive value. Some gauges are designed to measure negative pressure. For example, many physics experiments must take place in a vacuum chamber, a rigid chamber from which some of the air is pumped...

Research

JoVE Journal - Biology
Free Sample

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Cited by 267 •

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RNA transcripts are subject to extensive posttranscriptional regulation that is mediated by a multitude of trans-acting RNA-binding proteins (RBPs). Here we present a generalizable method to identify precisely and on a transcriptome-wide scale the RNA binding sites of RBPs.

Design Example: Strain Gauge Bridge or Wheatstone Bridge

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2024

The utilization of strain gauges as transducers for converting mechanical strain into electrical signals is a common practice in various engineering applications. These strain gauges are frequently integrated into Wheatstone bridge circuits to accurately measure parameters such as force or pressure. Within this context, each element within the circuit exhibits a resistance that undergoes subtle variations when subjected to mechanical strain. The primary objective is to convert minuscule...

In Situ Measurement of Vacuum Window Birefringence using 25Mg+ Fluorescence

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2020

Presented here is a method to measure the birefringence of vacuum windows by maximizing the fluorescence counts emitted by Doppler cooled 25Mg+ ions in an ion trap. The birefringence of vacuum windows will change the polarization states of the laser, which can be compensated by changing the azimuthal angles of external wave plates.

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Cited by 1 •

2016

Here we describe how to build a robust spring-transport mechanism for a spinning rotor gauge. This device securely immobilizes the rotor and keeps it under vacuum during transportation. We also describe packaging that minimizes the risk of damage during transport. Tests show our design works for typical shocks during transport.

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