Carotenoids

Carotenoids are a diverse group of lipid-soluble pigments found in plants, algae, fungi, and some microorganisms, where they support photosynthesis and protect cells from light-induced damage. Their extended system of conjugated double bonds absorbs specific wavelengths of light, enabling carotenoids to transfer excitation energy to chlorophyll and safely dissipate excess energy or quench reactive oxygen species. In biology, carotenoids contribute to the colors of leaves, flowers, fruits, and animals, while selected compounds serve as vitamin A precursors in animals. Studying carotenoid biosynthesis, transport, and metabolism informs research on plant adaptation, nutrition, vision, cellular protection, and biotechnology.

Carotenoids - Related Videos

Research

JoVE Journal - Medicine
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Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer

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Cited by 15 •

2020

We present a protocol to determine the levels of overall macular pigment, lutein, and zeaxanthin optical density in the central and parafoveal regions of the retina. The protocol includes a novel adjustable track system used to measure macular pigment optical density in the foveal eccentricity.

Education

JoVE Science Education - Environmental Sciences

Conversion of Fatty Acid Methyl Esters by Saponification for Uk'37 Paleothermometry

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2023

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds or, if interested in many, may need to remove unwanted constituents that are"in the way" or co-eluting. For example, the concentrations of individual compounds in a sample are often determined on a gas...

Photosynthesis - Concepts

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2019

Autotrophs Almost all living organisms on Earth depend on photosynthesis, which is the process that converts sunlight energy into a simple sugar called glucose. This molecule can be used as a short-term energy source or to build more complex carbohydrates like starches for long-term energy storage. Autotrophs are organisms that capture light energy using photosynthesis. Also known as primary producers, they provide the energy necessary to the organisms that eat them, which are known as...

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

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Cited by 2 •

2016

This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.

Reconstitution of Membrane Proteins

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2023

Reconstitution is the process of returning an isolated biomolecule to its original form or function. This is particularly useful for studying membrane proteins, which enable important cellular functions and affect the behavior of nearby lipids. To study the function of purified membrane proteins in situ, they must be reconstituted by integrating them into an artificial lipid membrane. This video introduces membrane protein reconstitution concepts and related procedures, such as protein...

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