In Vivo Delivery
In Vivo Delivery - Related Videos
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
16:19
Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Cited by 4 •
2013
Fluorescence sensors are powerful tools in life science. Here we describe a methodology to synthesize and use dendrimer-based fluorescent sensors to measure pH in living cells and in vivo. The dendritic scaffold enhances the properties of conjugated fluorescent dyes leading to improved sensing properties.
06:45
In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia Using a Custom-Built Delivery System
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Cited by 2 •
2020
This protocol presents techniques and methodology necessary for the accurate delivery of magnetic nanoparticle hyperthermia using a sophisticated delivery and monitoring system.
09:53
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
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Cited by 6 •
2018
Tissue-specific microRNA inhibition is a technology that is underdeveloped in the microRNA field. Herein, we describe a protocol to successfully inhibit the miR-181 microRNA family in myoblast cells from the heart. Nanovector technology is used to deliver a microRNA sponge that demonstrates significant in vivo cardio-specific miR-181 family inhibition.
11:12
Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
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Cited by 359 •
2013
A hemolysis assay can be used as a rapid, high-throughput screen of drug delivery systems' cytocompatibility and endosomolytic activity for intracellular cargo delivery. The assay measures the disruption of erythrocyte membranes as a function of environmental pH.
FAQs
Successful In Vivo Delivery depends on a sequence of biological events after administration. The delivered material must remain sufficiently stable, move through biological fluids, cross relevant tissue barriers, enter appropriate cells, and then be released or become active at the intended site. Failure at any stage can reduce the defined biological effect, even when the original payload is effective.
Biodistribution describes where the material travels throughout the organism, while targeting specificity concerns how selectively it reaches the intended tissue or cells. Stability determines whether the payload remains usable during transit, and immune or physiological responses can alter its persistence and activity. Together, these variables help explain why the same biological molecule, drug, gene, or cell may produce different outcomes in different delivery settings.
Injection, ingestion, and local administration create different starting points for transport through the organism. Consequently, the route influences which biological fluids and tissue barriers the material encounters before reaching its target. Route selection is therefore part of delivery design: researchers can relate the chosen administration path to the desired tissue, payload, and defined effect, then examine whether biodistribution and activity match that goal.
Immune and physiological responses can influence both the material and its destination. They may affect how long a payload remains available, whether it reaches the intended tissue, and how strongly the organism responds to it. Considering these responses is therefore essential when interpreting delivery outcomes in biology, particularly for approaches intended to change gene function, support therapy, or produce protective effects.
An effective strategy links four decisions: the biological payload, the intended target tissue, the administration route, and the effect to be measured. Researchers can then examine stability during transport, biodistribution, tissue-barrier passage, cellular uptake, release or activity, and immune or physiological responses. This sequence connects delivery design with evidence about whether the intended outcome occurred.
In biology, delivery approaches support several distinct goals. They can help researchers study gene function, evaluate therapeutic development, investigate vaccination, and advance regenerative medicine. The relevant outcome depends on whether the material reaches the intended tissue, remains stable, enters cells, and produces the planned activity, so delivery is part of the experimental design rather than a separate logistical step.
Delivery design is central to translation because laboratory findings must remain meaningful in a living organism. Assessing biodistribution, stability, targeting specificity, cellular uptake, release or activity, and immune or physiological responses shows whether a payload can produce its intended effect in biological conditions. These evaluations help connect experimental observations with potential clinical or broader biological applications.