Intraocular Pressure Measurement

Intraocular pressure measurement is the assessment of fluid pressure inside the eye, an important indicator of ocular health and a key tool for detecting pressure-related damage. Tonometry estimates this pressure by measuring the force required to flatten the cornea or the response of the cornea to a brief air pulse, with results influenced by corneal thickness and biomechanics. In biology and clinical research, these measurements support glaucoma screening, monitoring of disease progression, and evaluation of treatments that alter aqueous humor production or drainage. Reliable pressure assessment also helps researchers investigate how changes in eye physiology affect the optic nerve and visual function.

Intraocular Pressure Measurement - Related Videos

Research

JoVE EoE - Neuropathology

Induction of Elevated Intraocular Pressure in a Rat Model

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2025

Source: Lani-Louzada, R., et al., Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents. J. Vis. Exp. (2022)This video demonstrates the induction and monitoring of elevated intraocular pressure (IOP) in a rat model. The process involves cauterizing the limbal blood vessels, resulting in vessel occlusion and elevated IOP. The rat is then allowed to recover, and IOP is regularly monitored in both the experimental and control eyes for comparison.

Research

JoVE Journal - Behavior
Free Sample

Measuring the Behavioral Effects of Intraocular Scatter

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2021

In this protocol, we outline the conceptual design elements and structural development of a glare acuity apparatus. Additionally, the design of a device for measuring positive dysphotopsia (halos, spokes) and two-point light thresholds is described.

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs

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Cited by 59 •

2016

Rabbits are widely used to study the pharmacokinetics of intraocular drugs. We describe a method for conducting pharmacokinetic studies of intraocular drugs using rabbit eyes.

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

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Cited by 102 •

2016

This article describes a procedure for inducing retinal ischemia-reperfusion injury by elevated intraocular pressure in mice. Retinal ischemia-reperfusion injury by elevated intraocular pressure serves to model human pathologies characterized by compromised oxygen and nutrient delivery in the retina, enabling researchers to examine potential cellular mechanisms and treatments for human diseases of the retinal neurovascular unit.

Education

JoVE Science Education - Engineering

Noninvasive Blood Pressure Measurement Techniques

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2023

Source: Hamna J. Qureshi and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana Here we will highlight the key similarities and differences of noninvasive blood pressure measurement techniques between humans and rodents and examine the engineering principles that govern blood pressure. The principles that govern current cuff technology to acquire systolic and diastolic pressures will also be discussed. Commercially available cuffs that connect...

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