Loxp Sites
Loxp Sites - Related Videos
01:59
Cre-LoxP Mediated Induction of Cerebral Cavernous Malformations in a Mouse Model
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2025
In this video, a genetically modified mouse pup carrying the Ccm2 gene flanked by loxP sites and Cre recombinase fused to an estrogen receptor is used to induce cerebral cavernous malformations (CCMs). The administration of 4-hydroxytamoxifen activates Cre in endothelial cells, leading to the deletion of Ccm2 and the formation of CCMs in the brain.
03:12
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
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2025
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
01:49
Conserved Binding Sites
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2020
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function. Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
01:49
Conserved Binding Sites
0 Views •
2023
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function. Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
02:40
Ligand Binding Sites
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2020
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands. Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
FAQs
The relative orientation of two loxP sites directs the type of DNA rearrangement that occurs. When both sites face the same direction, Cre recombinase typically removes the DNA segment between them. When the sites face opposite directions, the intervening segment can be inverted instead. Thus, orientation provides a design principle for choosing deletion or inversion as the intended genetic outcome.
Flanking a regulatory region with loxP sites allows researchers to control more than the coding portion of a gene. Cre-mediated rearrangement can alter the regulatory DNA that influences gene activity, enabling studies of how that region contributes to gene function. This strategy is useful when the biological question concerns gene regulation rather than only the gene itself.
A conditional allele remains subject to Cre-mediated rearrangement until Cre is expressed in the relevant biological context. By selecting Cre expression in particular tissues, cell types, or developmental stages, researchers can restrict when and where the loxP-flanked DNA is rearranged. This conditional control helps connect a genetic change with a specific biological setting.
The design begins by placing loxP sites around the gene or regulatory region whose function will be examined. Researchers then use Cre expression in the selected tissue, cell type, or developmental stage to trigger rearrangement of the flanked DNA. The resulting allele supports a controlled genetic comparison in which gene function can be studied in a defined context.
This system supports conditional gene knockout, lineage tracing, genome engineering, and in vivo studies of gene function. Its value comes from linking a deliberate DNA rearrangement to selected biological contexts. Researchers can therefore examine consequences of gene loss, follow cellular lineages, or modify genomic regions while focusing on particular tissues, cell types, or developmental stages.
In lineage-tracing studies, Cre activity provides a way to associate a genetic rearrangement with selected cells or developmental contexts. A loxP-based design can therefore help researchers follow the descendants or biological outcomes connected with those cells. The approach is especially relevant when lineage behavior must be interpreted alongside tissue-specific or stage-specific gene activity in vivo.