Mirna Exchange
Mirna Exchange - Related Videos
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
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2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
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2023
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
11:38
Heat Exchanger Analysis
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2023
Source: Alexander S Rattner and Christopher J Greer; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA Heat exchangers transfer thermal energy between two fluid streams, and are ubiquitous in energy systems. Common applications include car radiators (heat transfer from hot engine coolant to surrounding air), refrigerator evaporators (air inside refrigerator compartment to evaporating refrigerant), and cooling towers in power plants...
02:06
Social Exchange Theory
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2020
We have discussed why we form relationships, what attracts us to others, and different types of love. But what determines whether we are satisfied with and stay in a relationship? One theory that provides an explanation is social exchange theory. According to social exchange theory, we act as naïve economists in keeping a tally of the ratio of costs and benefits of forming and maintaining a relationship with others (Rusbult & Van Lange, 2003). People are motivated to maximize the benefits...
FAQs
Packaging miRNAs into extracellular vesicles or other protective carriers helps support their movement through surrounding fluids. This carrier-based step connects production in one cell with delivery to another, making the molecules available for intercellular signaling. Examining carrier association therefore helps researchers investigate how miRNAs travel between cells and participate in biological communication.
After reaching a recipient cell, an miRNA can bind messenger RNA with a complementary sequence. This interaction alters gene expression in the receiving cell, extending the effect beyond the cell that produced the miRNA. The molecular outcome provides a direct link between miRNA delivery and changes in recipient-cell regulation.
The producing cell and recipient cell have different roles in this process. One cell supplies the miRNA, while another receives it and provides messenger RNA targets for the regulatory interaction. Distinguishing these roles shows how gene-regulatory effects can be transmitted between cells rather than remaining confined to the site of miRNA production.
A study can follow the process through linked stages: identify miRNAs associated with extracellular vesicles or other carriers, consider their movement through surrounding fluids, and examine their arrival in recipient cells. Researchers can then relate binding to complementary messenger RNAs with altered gene expression. This framework connects transport, delivery, and molecular outcome.
miRNA exchange provides context for studying regulation during development, immune responses, and tissue homeostasis. It is also relevant to disease-related signaling, where communication between cells may contribute to changes in biological behavior. Considering these settings helps researchers connect molecular RNA movement with larger processes that shape tissues and organismal function.
Because miRNAs can move between cells in protective carriers and occur in surrounding fluids, their exchange is relevant to biomarker investigations. Researchers can study exchanged miRNAs as molecular indicators associated with biological communication or disease-related signaling. This application extends the topic beyond mechanism by linking intercellular RNA movement with ways of characterizing biological states.
miRNA exchange informs investigation of RNA-based therapeutic strategies because it demonstrates how small RNAs can reach cells beyond their site of production and influence gene expression there. Understanding carrier-associated movement, delivery to recipient cells, and messenger RNA targeting can help frame research into approaches that use miRNA-related regulation for biological intervention.